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Transcription profiling of S. cerevisiae H2B K6,11,16,17,21,22G mutant vs. wild type to investigate the role of H2B N-terminal domain in transcription

ABSTRACT: Histone H2B was mutated to give H2B K6,11,16,17,21,22G. Total RNA from three replicate cultures of wild-type and mutant was isolated and the expression profiles were determined using Affymetrix arrays. Comparisons between the sample groups allow the identification of genes regulated by H2B N-terminal acetylated lysine residues. Experiment Overall Design: mutant compared to wild-type, 3 replicates

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: John Wyrick

PROVIDER: E-GEOD-3803 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deciphering the roles of the histone H2B N-terminal domain in genome-wide transcription.

Parra Michael A MA Kerr David D Fahy Deirdre D Pouchnik Derek J DJ Wyrick John J JJ

Molecular and cellular biology 20060501 10

Histone N-terminal domains are frequent targets of posttranslational modifications. Multiple acetylated lysine residues have been identified in the N-terminal domain of H2B (K6, K11, K16, K17, K21, and K22), but little is known about how these modifications regulate transcription. We systematically mutated the N-terminal domain of histone H2B, both at known sites of lysine acetylation and elsewhere, and characterized the resulting changes in genome-wide expression in each mutant strain. Our resu ...[more]

PMID: 16648479

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