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ATFS-1 mediates a protective transcription program during mitochondrial stress

ABSTRACT: ATFS-1 has been shown to regulate transcription of mitochondrial chaperone genes such as mtHsp70/hsp-6 and hsp-60 in response to mitocondrial stress. To identify the entire ATFS-1-mediated response, we compared the transcript profiles from wild-type and atfs-1(tm4525) worms raised in the absence and presence of mitochondrial stress. We used microarrays to identify genes regulated by ATFS-1 during mitochondrial stress RNA samples were prepared from wild-type(wt) and atfs-1(tm4525)(mutant) worms fed either control(RNAi) or spg-7(RNAi). Worms were synchronized by bleaching, raised in liquid culture and harvested at the L4 stage. Control is denoted as C and spg-7 treatment is denoted T. Each experiment was performed in triplicate indicated as 1,2 and 3.

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Jeffrey Zhao

PROVIDER: E-GEOD-38196 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mitochondrial import efficiency of ATFS-1 regulates mitochondrial UPR activation.

Nargund Amrita M AM Pellegrino Mark W MW Fiorese Christopher J CJ Baker Brooke M BM Haynes Cole M CM

Science (New York, N.Y.) 20120614 6094

To better understand the response to mitochondrial dysfunction, we examined the mechanism by which ATFS-1 (activating transcription factor associated with stress-1) senses mitochondrial stress and communicates with the nucleus during the mitochondrial unfolded protein response (UPR(mt)) in Caenorhabditis elegans. We found that the key point of regulation is the mitochondrial import efficiency of ATFS-1. In addition to a nuclear localization sequence, ATFS-1 has an N-terminal mitochondrial target ...[more]

PMID: 22700657

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