Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcriptional response to dengue infection in peripheral blood mononuclear cells of Nicaraguan children

ABSTRACT: We measured fever-day-specific genome-wide transcript abundance patterns in 105 peripheral blood mononuclear cell (PBMC) samples collected from 41 children hospitalized with dengue virus (DENV) infection in Nicaragua, and from 8 healthy controls. DF1 = primary DF; DF2 = secondary DF; DHF = Dengue Hemorrhagic Fever; DSS = Dengue Shock Syndrome. Samples were collected from pediatric patients with DENV infection at the Hospital Infantil Manuel de Jesus Rivera (HIMJR) in Managua, Nicaragua. Enrollment criteria consisted of hospitalized patients younger than 15 years of age whose parents or guardians completed the informed consent process, and for children 6 years and older who provided assent, and who presented with acute febrile illness of less than 7 days duration with one or more of the following symptoms or signs at the time of evaluation: headache, arthralgia, myalgia, retro-orbital pain, positive tourniquet test, petechiae, and signs of bleeding. Children were considered to have dengue if DENV was detected by RT-PCR or by isolation in cell culture, if an IgM ELISA indicated seroconversion between the acute and convalescent sample, and/or if there was a > 4-fold increase in DENV-specific antibodies between the acute and convalescent phase. Peripheral blood mononuclear cells (PBMCs) were isolated from whole blood within four hours of collection and stored in Trizol at -80 oC disease_state_design

ORGANISM(S): Homo sapiens

SUBMITTER: Stephen Popper

PROVIDER: E-GEOD-38246 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Temporal dynamics of the transcriptional response to dengue virus infection in Nicaraguan children.

Popper Stephen J SJ Gordon Aubree A Liu Minghsun M Balmaseda Angel A Harris Eva E Relman David A DA

PLoS neglected tropical diseases 20121220 12

Dengue is the most prevalent mosquito-borne human illness worldwide. The ability to predict disease severity during the earliest days of the illness is a long-sought, but unachieved goal.We examined human genome-wide transcript abundance patterns in daily peripheral blood mononuclear cell (PBMC) samples from 41 children hospitalized with dengue virus (DENV) infection in Nicaragua, as well as 8 healthy control subjects. Nine patients had primary dengue fever (DF1), 11 had dengue fever with serolo ...[more]

PMID: 23285306

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Dataset Information

Transcriptional response to dengue infection in peripheral blood mononuclear cells of Nicaraguan children

Publications

Temporal dynamics of the transcriptional response to dengue virus infection in Nicaraguan children.

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