Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Distinct Transcriptional Signatures of Bone Marrow-Derived C57BL/6 and DBA/2 Dendritic Leucocytes Hosting Live Leishmania amazonensis Amastigotes


ABSTRACT: To determine the modulation of gene expression of C57BL/6 and DBA/2 BMDLs in the presence of living intracellular Leishmania amazonensis amastigotes A genome-wide transcriptional analysis was performed by comparing the gene expression profiles of control DLs and live amastigote-hosting DLs from both mouse strains. Dendritic Leucocytes were generated in vitro from bone marrow progenitors (C57Bl/6 and DBA/2 mice). Leishmania amazonensis amastigotes were purified from mouse cutaneous lesions and were added to DL cultures. After 24h, and following a sorting procedure, only BMDls housing living amastigotes were selected for total RNA extraction. Three Biological replicates per condition were run.

ORGANISM(S): Mus musculus

SUBMITTER: Thierry Lang 

PROVIDER: E-GEOD-38261 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Distinct transcriptional signatures of bone marrow-derived C57BL/6 and DBA/2 dendritic leucocytes hosting live Leishmania amazonensis amastigotes.

Giraud Emilie E   Lecoeur Hervé H   Soubigou Guillaume G   Coppée Jean-Yves JY   Milon Geneviève G   Prina Eric E   Lang Thierry T  

PLoS neglected tropical diseases 20121213 12


<h4>Background/objectives</h4>The inoculation of a low number (10(4)) of L. amazonensis metacyclic promastigotes into the dermis of C57BL/6 and DBA/2 mouse ear pinna results in distinct outcome as assessed by the parasite load values and ear pinna macroscopic features monitored from days 4 to 22-phase 1 and from days 22 to 80/100-phase 2. While in C57BL/6 mice, the amastigote population size was increasing progressively, in DBA/2 mice, it was rapidly controlled. This latter rapid control did not  ...[more]

Similar Datasets

2013-01-07 | GSE38261 | GEO
2010-06-15 | E-GEOD-16644 | biostudies-arrayexpress
2010-06-15 | GSE16644 | GEO
2020-06-11 | GSE144039 | GEO
2009-01-01 | GSE11497 | GEO
2009-01-14 | E-GEOD-11497 | biostudies-arrayexpress
2012-12-15 | E-GEOD-41641 | biostudies-arrayexpress
2012-12-15 | GSE41641 | GEO
2005-12-20 | GSE3835 | GEO
| PRJNA167502 | ENA