Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional analysis of fluorescence-activated sorted Mesp-GFP-labeled B7.5 founder trunk ventral cell lineage cells from Ciona intestinalis


ABSTRACT: The Ciona heart progenitor lineage (TVC, trunk ventral cells) is first specified by Fibroblast Growth Factor/Map Kinase (FGF/MapK) activation of the transcription factor Ets1/2 (Ets). For this analysis, B7.5 lineage cells were labeled with the Mesp-GFP reporter. In the first experiment, we targeted a dominant-negative form of the sole Ciona FGF receptor (FGFRdn) to the B7.5 lineage using the Mesp enhancer (Mesp-FGFRdn). In the second experiment, we targeted a dominant repressor form of Ets1/2 to the B7.5 lineage using the Mesp enhancer (Mesp-EtsWRPW). This construct is designed to repress Ets1/2 target gene transcription and has previously been shown to abolish TVC induction. We employed whole-genome microarray analysis of sorted B7.5 lineage cells to identify primary FGF:MapK:Ets1/2 target genes.

ORGANISM(S): Ciona intestinalis

SUBMITTER: Bradley Davidson 

PROVIDER: E-GEOD-38313 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Initial deployment of the cardiogenic gene regulatory network in the basal chordate, Ciona intestinalis.

Woznica Arielle A   Haeussler Maximilian M   Starobinska Ella E   Jemmett Jessica J   Li Younan Y   Mount David D   Davidson Brad B  

Developmental biology 20120514 1


The complex, partially redundant gene regulatory architecture underlying vertebrate heart formation has been difficult to characterize. Here, we dissect the primary cardiac gene regulatory network in the invertebrate chordate, Ciona intestinalis. The Ciona heart progenitor lineage is first specified by Fibroblast Growth Factor/Map Kinase (FGF/MapK) activation of the transcription factor Ets1/2 (Ets). Through microarray analysis of sorted heart progenitor cells, we identified the complete set of  ...[more]

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