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Single-cell RNA-seq of the 16-cell stage Ciona embryo

ABSTRACT: Ciona develops according to a stereotyped or invariant lineage. This allowed us to collect precisely defined replicates of all eight blastomeres of the right half of the embryo at the 16-cell stage of Ciona. We manually dissected each embryo, collecting and identifying each cell before library preparation and single-cell RNA-seq to give us a data set of 8 blastomeres x 4 embryos (32 cells). We sequenced these on MiSeq and HiSeq 2500. A fifth embryo was only sequenced on MiSeq and excluded from subsequent analysis.

INSTRUMENT(S): Illumina HiSeq 2500, Illumina MiSeq

ORGANISM(S): Ciona robusta

SUBMITTER: Garth Ilsley

PROVIDER: E-MTAB-6117 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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