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Genome-wide effects of Pbcas4 knockdown

ABSTRACT: We tested the effect iof Pbcas4 knockdown using a specific shRNA on the expression of genes sharing miRNA binding sites in mouse N2A cells. 2 samples (shRNA and scramble), 3 biological replicates for each sample

ORGANISM(S): Mus musculus

SUBMITTER: Ana Marques

PROVIDER: E-GEOD-38333 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Evidence for conserved post-transcriptional roles of unitary pseudogenes and for frequent bifunctionality of mRNAs.

Marques Ana C AC Tan Jennifer J Lee Sheena S Kong Lesheng L Heger Andreas A Ponting Chris P CP

Genome biology 20121115 11

<h4>Background</h4>Recent reports have highlighted instances of mRNAs that, in addition to coding for protein, regulate the abundance of related transcripts by altering microRNA availability. These two mRNA roles - one mediated by RNA and the other by protein - are inter-dependent and hence cannot easily be separated. Whether the RNA-mediated role of transcripts is important, per se, or whether it is a relatively innocuous consequence of competition by different transcripts for microRNA binding ...[more]

PMID: 23153069

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