The maize silks transcriptome
Ontology highlight
ABSTRACT:
ORGANISM(S): Zea mays
SUBMITTER: Maria Mejia Guerra
PROVIDER: E-GEOD-38414 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
A genome-wide regulatory framework identifies maize pericarp color1 controlled genes.
Morohashi Kengo K Casas María Isabel MI Falcone Ferreyra Maria Lorena ML Falcone Ferreyra Lorena L Mejía-Guerra María Katherine MK Pourcel Lucille L Yilmaz Alper A Feller Antje A Carvalho Bruna B Emiliani Julia J Rodriguez Eduardo E Pellegrinet Silvina S McMullen Michael M Casati Paula P Grotewold Erich E
The Plant cell 20120720 7
Pericarp Color1 (P1) encodes an R2R3-MYB transcription factor responsible for the accumulation of insecticidal flavones in maize (Zea mays) silks and red phlobaphene pigments in pericarps and other floral tissues, which makes P1 an important visual marker. Using genome-wide expression analyses (RNA sequencing) in pericarps and silks of plants with contrasting P1 alleles combined with chromatin immunoprecipitation coupled with high-throughput sequencing, we show here that the regulatory functions ...[more]
Similar Datasets
Project description:P1 encodes an R2R3-MYB transcription factor responsible for the accumulation of insecticidal flavones in maize silks and red phlobaphene pigments in pericarps and other floral tissues, which contributed to making P1 an important visual marker since the dawn of modern genetics. We conducted RNA-Seq using pericarps at two different stages, 14 and 25 days after pollination (DAP). High-throughput sequencing using the Illumina platform resulted in the generation of ~20 million high quality reads, from which ~90% aligned to the recently completed maize genome sequence corresponding to ~5 million reads for each one of the four samples. Examination of two different RNA samples from two different stages of maize pericarp tissues.
2012-09-14 | E-GEOD-38413 | biostudies-arrayexpress
Project description:To determine which of the genes differentially expressed between P1-rr and P1-ww pericarps were immediate (direct) targets of P1, we conducted chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-Seq) using P1 polyclonal antibodies (alphaP1344) that recognize the non-conserved C-terminal region of P1 (Falcone Ferreyra et al., 2010), on pericarp chromatin. Comparison of pericarp chromatin inmunoprecipitated material P-rr_14DAP (P1 expressed) vs P-ww_14DAP (P1 not expressed) to determine P1 direct targets
2012-09-14 | E-GEOD-38587 | biostudies-arrayexpress
Project description:P1 encodes an R2R3-MYB transcription factor responsible for the accumulation of insecticidal flavones in maize silks and red phlobaphene pigments in pericarps and other floral tissues, which contributed to making P1 an important visual marker since the dawn of modern genetics. We conducted RNA-Seq using from maize silks obtained at 2-3 days after emergence. High-throughput sequencing using the Illumina platform resulted in the generation of ~14 million high quality reads, corresponding to ~7 million reads for each sample, from which 76% aligned to the maize genome.
2012-09-14 | GSE38414 | GEO
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2013-08-19 | E-GEOD-46167 | biostudies-arrayexpress
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2008-04-01 | E-GEOD-10894 | biostudies-arrayexpress
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Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series
2013-06-27 | E-GEOD-44862 | biostudies-arrayexpress