Ontology highlight
ABSTRACT:
ORGANISM(S): Schizosaccharomyces pombe
SUBMITTER: Mario Halic
PROVIDER: E-GEOD-38636 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Marasovic Mirela M Zocco Manuel M Halic Mario M
Molecular cell 20131003 2
RNAi is a conserved mechanism in which small RNAs induce silencing of complementary targets. We have previously identified priRNAs, a class of Dicer-independent small RNAs in fission yeast. The mechanism by which Dicer-independent small RNAs are generated is not well understood in any species. Here we reconstitute the final steps of priRNA and siRNA biogenesis in vitro. We identify the 3'-5' exonuclease Triman and demonstrate that Argonaute, loaded with longer RNA precursors, recruits Triman to ...[more]