Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Argonaute and Trimmer generate Dicer-independent priRNAs and nucleate RNAi


ABSTRACT: RNA interference is involved in silencing of transposable and repetitive elements. How these elements are initially recognized by RNAi is a fundamental and unanswered question. We previously identified a class of Dicer-independent small RNAs, called primal small RNAs (priRNAs), in fission yeast. The mechanism by which Dicer-independent small RNAs are generated is not clear for any species. Here we reconstitute priRNA biogenesis in vitro and demonstrate that priRNAs can nucleate RNAi in vivo. We identify 3'-5' exonuclease Trimmer and show that Argonaute, loaded with longer RNA precursors, recruits Trimmer to generate the new 3' end. Next, we show that antisense priRNAs accumulate in rrp6M-NM-^T cells and nucleate RNAi at subset of protein coding genes in a Trimmer- and priRNA-dependent manner. Thus, Rrp6-mediated degradation of antisense transcripts and priRNA precursors protects the genome from spurious RNAi. Our results suggest that Argonaute association with random RNA degradation products triggers RNAi in a process of transcriptome surveillance. small RNA profiling in wild type S. pombe cells and in mutant cells

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Mario Halic 

PROVIDER: E-GEOD-38636 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Argonaute and Triman generate dicer-independent priRNAs and mature siRNAs to initiate heterochromatin formation.

Marasovic Mirela M   Zocco Manuel M   Halic Mario M  

Molecular cell 20131003 2


RNAi is a conserved mechanism in which small RNAs induce silencing of complementary targets. We have previously identified priRNAs, a class of Dicer-independent small RNAs in fission yeast. The mechanism by which Dicer-independent small RNAs are generated is not well understood in any species. Here we reconstitute the final steps of priRNA and siRNA biogenesis in vitro. We identify the 3'-5' exonuclease Triman and demonstrate that Argonaute, loaded with longer RNA precursors, recruits Triman to  ...[more]

Similar Datasets

2010-02-23 | E-GEOD-19734 | biostudies-arrayexpress
2008-11-30 | E-GEOD-12014 | biostudies-arrayexpress
2013-06-05 | E-GEOD-47650 | biostudies-arrayexpress
2011-01-14 | E-GEOD-25897 | biostudies-arrayexpress
2012-11-28 | E-GEOD-35637 | biostudies-arrayexpress
2012-11-28 | E-GEOD-35638 | biostudies-arrayexpress
2013-05-01 | E-GEOD-45974 | biostudies-arrayexpress
2014-04-01 | E-GEOD-53273 | biostudies-arrayexpress
2014-10-30 | E-GEOD-27269 | biostudies-arrayexpress
2015-03-01 | E-GEOD-64429 | biostudies-arrayexpress