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Agilent data from Arabidopsis thaliana enriched guard cell tissue and leafs.

ABSTRACT: To identify genes of the guard cell transkriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity. Ost1-2 and slac1-3 mutants were compared to their wildtype. total samples analysed are 35: 4 biolocigal independent replicates of: total leaf (COL-0) vs. enriched guard cells (COL-0); ABA-sprayed enriched guard cells (gl1-1) vs. control-sprayed enriched guard cells (gl1-1); enriched guard cells (slac1-3) vs. enriched guard cells (gl1-1);guard cells (ost1-2) vs. guard cells (ler);low humidity(20%rh) treated enriched guard cells (COL-0) vs. high humidity(80%) treated enriched guard cells (COL0)

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Susanne Kneitz

PROVIDER: E-GEOD-38639 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Agilent data from Arabidopsis thaliana enriched guard cell tissue and leafs.

Project description:To identify genes of the guard cell transcriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity. total samples analysed are 24: 4 biological independent replicates of: total leaf (COL-0) vs. enriched guard cells (COL-0); ABA-sprayed enriched guard cells (gl1-1) vs. control-sprayed enriched guard cells (gl1-1); low humidity (20%rh) treated enriched guard cells (COL-0) vs. high humidity (80%) treated enriched guard cells (COL-0)

2013-01-14 | E-GEOD-41054 | biostudies-arrayexpress

Agilent data from Arabidopsis thaliana enriched guard cell tissue of ost1-2,slac 1-5, gl1-1 and landsberg erecta

Project description:To identify genes of the guard cell transcriptome of Arabidopsis thaliana enriched guard cell samples of ost1-2 and slac1-5 mutants were compared to their wildtype. Total samples analysed are 11: biological independent replicates of: enriched guard cells (slac1-5) vs. enriched guard cells (gl1-1); enriched guard cells (ost1-2) vs. guard cells (ler);

2013-11-20 | E-GEOD-42470 | biostudies-arrayexpress

Agilent data from Arabidopsis thaliana enriched guard cell tissue and leafs.

2013-01-14 | GSE41054 | GEO

Agilent data from Arabidopsis thaliana enriched guard cell tissue and leafs.

Project description:To identify genes of the guard cell transkriptome of Arabidopsis thaliana enriched guard cell samples were compared with total leaf tissue. Genes of the abscisic acid and humidity response of Arabidopsis thaliana guard cells were identified by treatment with ABA-Spray and low humidity. Ost1-2 and slac1-3 mutants were compared to their wildtype.

2013-06-04 | GSE38639 | GEO

Agilent data from Arabidopsis thaliana enriched guard cell tissues (GC) treated with high CO2 and darkness

Project description:Genes of the of Arabidopsis thaliana guard cells transcriptome that respond to high CO2 and darkness were identified and compared to the ABA- and low humidity treated samples of Experiment GSE41054 in Arabidopsis thaliana enriched guard cell samples.

2019-07-01 | GSE118520 | GEO

maternal/embryonic aba-1-Analysis of the role of maternal and embryonic ABA in seeds.

Project description:ra07-02_aba2 - maternal/embryonic aba-1 - Identification of genes regulated by maternal and embryonic ABA - Comparison of expression in seeds (15 days after pollination) of aba2-2 mutant to wild type (Col-0), aba2 (female) x Col-0 (male) crossing, and aba2-2 sprayed with ABA (100 micro M, twice a week). Keywords: gene knock out,treated vs untreated comparison

2008-05-29 | GSE10674 | GEO

Transcription profiling by array of Arabidopsis guard cells and mesophyll cells in response to ABA treatment

Project description:Transcriptional profiling of guard cells and mesophyll cells in response to ABA treatment

2008-02-02 | E-MEXP-1443 | biostudies-arrayexpress

Expression data from Arabidopsis leaves

Project description:Flavonols are a class of flavonoids. Unlike other classes of flavonoids, flavonols strongly accumulate in guard cells, but the role of their accumulation in guard cells is still unclear so far.The overexpression of TaFLS1, a flavonol synthase gene from wheat, improves flavonol content in guard cells of Arabidopsis, while the mutant fls1-3 of Arabidopsis AtFLS1 reduces the content. Both the increase of flavonols by TaFLS1 overexpression and the decrease in fls1-3 change stomatal apeture, showing the association between flavonols and stomatal movements. We used microarrays to detail the global programme of gene expression underlying both the increase and decrease of flavonol content and identified possible mechanism of flavonols-mediated stomatal movement. Arabidipsis Col-0, TaFLS1 overexpression lines, and fls1-3 were used for analysis. Leaves of six-week old Arabidopsis seedlings grown in soils under the regime of a 16 h photoperiod at 22°C and 60% relative humidity were sampled, and RNA was extracted for microarray analysis. Each of Arabidopsis lines has three replicates.

2014-02-01 | E-GEOD-54593 | biostudies-arrayexpress

Gene expression in the epidermis including guard cells of phot1 phot2 and pGC1::SOC1-GFP/phot1 phot2

Project description:Stomata pores are surrounded by a pair of guard cells in the epidermis in plants. Stomata apature is controlled by environmental factors. Stomata opening is also enhanced by introduction of SOC1-GFP driven by GC1 promoter in phot1 phot2 double mutants (pGC1::SOC1-GFP/ phot1 phot2). Epidermis including guard cells were isolated from phot1 phot2 and pGC1::SOC1-GFP/phot1 phot2 and gene expression were analyzed. phot1-5 phot2-1 in gl1 background (phot1 phot2) and pGC1::SOC1-GFP/phot1 phot2 were grown under 16 h light / 8h dark, constant 22?C conditions for 4 to 5 weeks. Epidermis including guard cells were isolated from leaves of these plants. Three biological replicates were used.

2015-02-04 | E-GEOD-60183 | biostudies-arrayexpress

Genome-wide GL3 and GL1 binding sites in Arabidopsis thaliana

Project description:GLABRA3 (GL3) and GLABRA1 (GL1) function as selector genes that control the differentiation of a group of protodermal cells into trichomes in Arabidopsis thaliana. We performed genome-wide location (ChIP-chip) analyses by using transgenic Arabidopsis plants carrying GL3-YFP or GL1-YFP-MYC1 mini genes. These analyses identified statistically significant enriched DNA associated with GL1 and GL3. A total ~540 and ~700 genes were identified as located proximal and downstream to the GL1 and GL3 binding regions, respectively. Keywords: ChIP-chip

2009-03-02 | GSE13090 | GEO

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