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Macrophage samples from the HMDP

ABSTRACT: Identify genes involved in regulation of inflammatory responses and gene-environemnt interactions, in macrophages from a set of mouse inbred strains termed the HMDP. The HMDP is a genetically diverse mapping panel comprised of classical inbred and recombinant inbred wild type mice. The RMA values of genes were used for genome wide association as described in Bennett et al Genome Research 2010. GWAS for expression in primary macrophages of mouse inbred strains. Macrophages were exposed to bacterial lipopolysaccharide (LPS), oxidized lipids (OxPAPC) or control treatment conditions.

ORGANISM(S): Mus musculus

SUBMITTER: Calvin Pan

PROVIDER: E-GEOD-38705 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Unraveling inflammatory responses using systems genetics and gene-environment interactions in macrophages.

Orozco Luz D LD Bennett Brian J BJ Farber Charles R CR Ghazalpour Anatole A Pan Calvin C Che Nam N Wen Pingzi P Qi Hong Xiu HX Mutukulu Adonisa A Siemers Nathan N Neuhaus Isaac I Yordanova Roumyana R Gargalovic Peter P Pellegrini Matteo M Kirchgessner Todd T Lusis Aldons J AJ

Cell 20121001 3

Many common diseases have an important inflammatory component mediated in part by macrophages. Here we used a systems genetics strategy to examine the role of common genetic variation in macrophage responses to inflammatory stimuli. We examined genome-wide transcript levels in macrophages from 92 strains of the Hybrid Mouse Diversity Panel. We exposed macrophages to control media, bacterial lipopolysaccharide (LPS), or oxidized phospholipids. We performed association mapping under each condition ...[more]

PMID: 23101632

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