Unknown,Transcriptomics,Genomics,Proteomics

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Skeletal muscle and insulin regulated genes

ABSTRACT: Insulin action in target tissues involved precise regulation of gene expression. To define the set of insulin-regulated genes in human skeletal muscle, we analyzed the global changes in mRNA levels during a 3-h hyperinsulinemic euglycemic clamp in vastus lateralis muscle of six healthy subjects. Using 29,308 cDNA element microarrays, we found that the mRNA expression of 762 genes, including 353 expressed sequence tags, was significantly modified during insulin infusion. 478 were up-regulated and 284 down-regulated. Most of the genes with known function are novel targets of insulin. They are involved in the transcriptional and translational regulation (29%), intermediary and energy metabolisms (14%), intracellular signaling (12%), and cytoskeleton and vesicle traffic (9%). Other categories consisted of genes coding for receptors, carriers, and transporters (8%), components of the ubiquitin/proteasome pathways (7%) and elements of the immune response (5.5%). These results thus define a transcriptional signature of insulin action in human skeletal muscle. They will help to better define the mechanisms involved in the reduction of insulin effectiveness in pathologies such as type 2 diabetes mellitus, a disease characterized by defective regulation of gene expression in response to insulin. An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Using regression correlation

ORGANISM(S): Homo sapiens

SUBMITTER: Karine Clement

PROVIDER: E-GEOD-3881 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray profiling of human skeletal muscle reveals that insulin regulates approximately 800 genes during a hyperinsulinemic clamp.

Rome Sophie S Clément Karine K Rabasa-Lhoret Rémi R Loizon Emmanuelle E Poitou Christine C Barsh Greg S GS Riou Jean-Paul JP Laville Martine M Vidal Hubert H

The Journal of biological chemistry 20030305 20

Insulin action in target tissues involved precise regulation of gene expression. To define the set of insulin-regulated genes in human skeletal muscle, we analyzed the global changes in mRNA levels during a 3-h hyperinsulinemic euglycemic clamp in vastus lateralis muscle of six healthy subjects. Using 29,308 cDNA element microarrays, we found that the mRNA expression of 762 genes, including 353 expressed sequence tags, was significantly modified during insulin infusion. 478 were up-regulated and ...[more]

PMID: 12621037

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Dataset Information

Skeletal muscle and insulin regulated genes

Publications

Microarray profiling of human skeletal muscle reveals that insulin regulates approximately 800 genes during a hyperinsulinemic clamp.

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