Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Expression data from Arabidopsis petioles in early stage dense canopy

ABSTRACT: Growth in dense stands induces shade avoidance responses. Early responses to neighbors seem to be assoctaed with touch, not light signalling. We studied gene expression in petioles during early canopy development when leaf hyponasty was visible but altered phytochrome signalling was not yet detectable. Plants were grown in small pots that were either kept single, or put in a high density of 2066 plants/m2. Gene expression measured in petioles when canopies had reached a leaf area index of 0.9.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ronald Pierik

PROVIDER: E-GEOD-39010 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Plant neighbor detection through touching leaf tips precedes phytochrome signals.

de Wit Mieke M Kegge Wouter W Evers Jochem B JB Vergeer-van Eijk Marleen H MH Gankema Paulien P Voesenek Laurentius A C J LA Pierik Ronald R

Proceedings of the National Academy of Sciences of the United States of America 20120820 36

Plants in dense vegetation compete for resources, including light, and optimize their growth based on neighbor detection cues. The best studied of such behaviors is the shade-avoidance syndrome that positions leaves in optimally lit zones of a vegetation. Although proximate vegetation is known to be sensed through a reduced ratio between red and far-red light, we show here through computational modeling and manipulative experiments that leaves of the rosette species Arabidopsis thaliana first ne ...[more]

PMID: 22908260

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Project description:Plants grown under a canopy recognize changes in light quality and modify their growth patterns; this modification is known as shade avoidance syndrome. In leaves, leaf blade expansion is suppressed, whereas petiole elongation is promoted under the shade. However, the mechanisms that control these responses are largely unclear. Here, we demonstrated that both auxin and brassinosteroid (BR) are required for the normal leaf responses to shade. The microarray analysis of leaf blades and petioles treated with end-of-day far-red light (EODFR) revealed that almost half of the genes induced by the treatment in both parts were previously identified as auxin-responsive genes. Likewise, BR-responsive genes were overrepresented in the EODFR-induced genes. Hence, the auxin and BR responses were elevated by EODFR treatment in both leaf blades and petioles, although opposing growth responses were observed in these two parts. The analysis of the auxin-deficient doc1/big mutant and BR-deficient rot3/cyp90c1 mutant further indicates that auxin and BR were equally required for the normal petiole elongation response to the shade stimulus. In addition, the spotlight irradiation experiment revealed that phytochrome in leaf blades but not that in petioles regulated petiole elongation, which was probably mediated through regulation of the auxin/BR responses in petioles. On the basis of these findings, we conclude that auxin and BR cooperatively promote petiole elongation in response to the shade stimulus under the control of phytochrome in the leaf blade. The WT seedlings were grown for 19 days under continuous white light condition before experimental treatment with three light conditions. Seedlings were either maintained in white light for 2 h (WL), incubated in the dark condition for 2 h (D), or experienced a pulse irradiation of FR light before incubated in the dark condition for 2 h (FRD). Total RNAs were separately prepared from leaf blades and petioles after each light treatment. Three independent biological replicates were used.

Dataset Information

Expression data from Arabidopsis petioles in early stage dense canopy

Publications

Plant neighbor detection through touching leaf tips precedes phytochrome signals.

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