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Transcription profiling of Arabidopsis response to sucrose feeding to detached leaves

ABSTRACT: The effect of sucrose feeding on gene expression in Arabidopsis thaliana leaves was investigated using affymetrix ATH1 microarrays. For this, petioles of detached leaves were put in a solution containing either sucrose or sorbitol (control). Sugars were taken up into the leaf via the respiration stream for 13 hours. After that, leaves were frozen in liquid nitrogen and RNA was extracted for analysis. Experiment Overall Design: The effect of sucrose feeding on gene expression was investigated using affymetrix ATH1 microarrays. For this, petioles of detached leaves were put in a solution containing either sucrose or sorbitol (control). Sugars were taken up into the leaf via the respiration stream for 13 hours. After that, leaves were frozen in liquid nitrogen and RNA was extracted for analysis.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Joost van Dongen

PROVIDER: E-GEOD-4022 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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