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Difference expression analyses before and after human fucosyltransferase gene 1 transfection into ovarian cancer clear cell line (Human cancer array)

ABSTRACT: 75% ovarian epithelial tumor overexpresses Lewis y antigen, which notably strenthen such malignant biological behaviors as metastasis, drug resistance and proliferation. The key enzyme of Lewis y synthesis is human fucosyltransferase gene 1(hfut1). Through transfecting hfut1 into Lewis y low expressing cell line RMG-I, we got a pair of cell lines. We hypothesized that Lewis y antigen plays a major role in ovarian canceration . Using gene arrays, we comprehensively profiled mRNA differential expression between fut1-pretransfected and posttransfected clear cell of human epithelial OvCa. We expected that over-expression of lewis y antigen would lead to higher expression of genes associated with growth, metastasis,proliferation and so on. we established ovarian tumor cell line RMG-I-H by stably transfecting human fucosyltransferase gene 1 into cell line RMG-I. RMG-I-H overexpresses Lewis y antigen, which is known as a cancer-related tetrasaccharide antigen. We profiled for tumor-associated gene expression both cells pretransfected and posttransfected.

ORGANISM(S): Homo sapiens

SUBMITTER: Ziwei Feng

PROVIDER: E-GEOD-39216 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Difference expression analyses before and after human fucosyltransferase gene 1 transfection into ovarian cancer clear cell line

Project description:75% ovarian epithelial tumor overexpresses Lewis y antigen, which notably strenthen such malignant biological behaviors as metastasis, drug resistance and proliferation. The key enzyme of Lewis y synthesis is human fucosyltransferase gene 1(hfut1). Through transfecting hfut1 into Lewis y low expressing cell line RMG-I, we got a pair of cell lines. We hypothesized that Lewis y antigen plays a major role in ovarian canceration . Using gene arrays, we comprehensively profiled mRNA differential expression between fut1-pretransfected and posttransfected clear cell of human epithelial OvCa. We expected that over-expression of lewis y antigen would lead to higher expression of genes associated with growth, metastasis,proliferation and so on. we established ovarian tumor cell line RMG-I-H by stably transfecting human fucosyltransferase gene 1 into cell line RMG-I. RMG-I-H overexpresses Lewis y antigen, which is known as a cancer-related tetrasaccharide antigen. We profiled for gene expression both cells pretransfected and posttransfected.

2012-08-01 | E-GEOD-34396 | biostudies-arrayexpress

Difference expression analyses before and after human fucosyltransferase gene 1 transfection into ovarian cancer clear cell line

2012-08-02 | GSE34396 | GEO

Difference expression analyses before and after human fucosyltransferase gene 1 transfection into ovarian cancer clear cell line (Human cancer array)

2012-08-02 | GSE39216 | GEO

Gene expression profiles of control GC424 tumor cell lines and after downregulation of the SV40 large T-antigen

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GC424 tumor cells and gastric tumors

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Monocyte-like THP-1 Cells: Trichosporon asahii Infected vs Control

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An embeddable molecular code for Lewis X modification through interaction with fucosyltansferase 9

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Human umbilical vascular endothelial cells: control vs. safrole oxide treatment

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Adipose-derived stem cells improved mouse ovary function after chemotherapy-induced ovary failure

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2013-06-11 | E-GEOD-47741 | biostudies-arrayexpress

Transcription profiling of mouse bone marrow-derived dendritic cells (BMDC), with cells that had been cocultured (1:5, tumor:BMDC) with mouse ovarian surface epithelial cell line (1D8) cells, with irradiated (50Gy) 1D8 cells, or with the supernatent from 1D8 cells (25%, v/v)

Project description:The Suppressor of cytokine signaling (SOCS) family of negative regulatory proteins are upregulated in response to several cytokines and pathogen-associated molecular patterns (PAMPs), and suppress cellular signaling responses by binding receptor phosphotyrosine residues. Exposure of bone marrow-derived dendritic cells (BMDCs) to 1D8 cells, a murine model of ovarian carcinoma, suppresses their ability to express CD40 and stimulate antigen specific responses in response to PAMPs, and in particular to poly I: C with the upregulated SOCS3 transcript and protein levels. The ectopic expression of SOCS3 in both the macrophage cell line RAW264.7 and BMDCs decreased signaling in response to both poly I:C and IFNÎ±. Further, knockdown of SOCS3 transcripts significantly enhanced the responses of RAW264.7 and BMDCs to both poly I: C and IFNÎ±. Immunoprecipitation and pull-down studies demonstrate that SOCS3 binds to the IFNÎ± receptor TYK2. Since poly I: C triggers autocrine IFNÎ± signaling, binding of SOCS3 to TYK2 may thereby suppress the activation of BMDCs by polyI:C and IFNÎ±. Thus, elevated levels of SOCS3 in tumor-associated DCs may potentially resist the signals induced by TLR3 ligands and type I interferon to decrease DC activation via binding with IFNÎ± receptor TyK2. Experiment Overall Design: Microarray analysis was used to compare the expression levels of Control mouse bone marrow-derived dendritic cells (BMDC), with cells that had been cocultured (1:5, tumor:BMDC) with mouse ovarian surface epithelial cell line (1D8) cells, with irradiated (50Gy) 1D8 cells, or with the supernatent from 1D8 cells (25%, v/v). Experiment Overall Design: One biologic sample was analyzed for each condition, four samples in all.

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