Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Expression data from murine intestinal tissue upon depletion of regulatory T cells

ABSTRACT: Regulatory T cells (Treg) play a pivotal role in modulating immune responses and were shown to decrease atherosclerosis in murine models. How this effect is brought about remains elusive. We used microarrays to detail the global programme of gene expression in intestinal tissue from chimeric DEREG x Ldlr-/- mice +/- diphteria toxin enabling selective depletion of Treg. Chimeric DEREG x Ldlr-/- mice were kept on a high fat diet for 8 weeks and treated with DT or PBS i.p. for 8 weeks. Upon harvest, intestinal tissue was ascertained from animals from both groups and a gene array was performed.

ORGANISM(S): Mus musculus

SUBMITTER: Stefan Zoller

PROVIDER: E-GEOD-39351 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Expression data from murine liver tissue upon depletion of regulatory T cells

Project description:Regulatory T cells (Treg) play a pivotal role in modulating immune responses and were shown to decrease atherosclerosis in murine models. How this effect is brought about remains elusive. We used microarrays to detail the global programme of gene expression in liver tissue from chimeric DEREG x Ldlr-/- mice +/- diphteria toxin enabling selective depletion of Treg. Chimeric DEREG x Ldlr-/- mice were kept on a high fat diet for 8 weeks and treated with DT (vehicle) or PBS (placebo) i.p. for 8 weeks. Upon harvest, liver tissue was ascertained from animals from both groups and a gene array was performed.

2012-12-31 | E-GEOD-36279 | biostudies-arrayexpress

Expression data from murine intestinal tissue upon depletion of regulatory T cells

2013-07-10 | GSE39351 | GEO

Comparison of expression profiles of Foxp3(+)epigenetics(-) T cells, Foxp3(-)epigenetics(+) T cells, and Foxp3(+)epigenetics(+) T cells

Project description:Analysis of Foxp3(+)epigenetics(-) T cells, Foxp3(-)epigenetics(+) T cells, and Foxp3(+)epigenetics(+) T cells. Results indicate regulatory T cell (Treg) ontogenesis requires two independent processes, expression of the transcription factor Foxp3 and establishment of Treg epigenetic programs induced by T cell receptor (TCR) stimulation. GFP+CD4+ and GFP-CD4+ splenocytes were sorted from DEREG and DEREG/Scurfy mice. These cells were activated with anti-CD3/CD28 antibodies, and then transduced with Foxp3-expressing retrovirus (pGCSamIN, NGFR marker). NGFR+ T cells sorted were subjected to microarray analysis (Affymetrix, mouse genome 430 2.0 array). To normalize the experimental conditions, Tregs (GFP+ T cells from DEREG) and Tconv (GFP- T cells from DEREG) were also activated and transduced with empty vector. Two replicates each.

2012-11-05 | E-GEOD-25252 | biostudies-arrayexpress

Expression data from murine liver tissue upon depletion of regulatory T cells

2012-12-31 | GSE36279 | GEO

Gene expression data from mouse regulatory T cells

Project description:Intravenous Immunoglobulin (IVIg) is widely used as an immunomodulatory therapy. We have recently demonstrated that IVIg protects against airway hyper-reactivity (AHR) and inflammation in mouse models of allergic airway disease (AAD), associated with induction of Foxp3+ regulatory T cells (Treg). Using DEREG (DEpletion of REGulatory T cell) mice, in which endogenous Treg can be ablated with Diphtheria toxin (DTx) treatment, we demonstrate that IVIg generates a de novo population of induced Treg (iTreg) in the absence of endogenous Treg. IVIg-generated iTreg were sufficient for inhibition of ovalbumin-induced AHR in an antigen-driven murine model of AAD. In the absence of endogenous Treg, IVIg failed to confer protection against AHR and airway inflammation. Adoptive transfer of purified IVIg-generated iTreg prior to antigen challenge effectively prevented airway inflammation and AHR in an antigen-specific manner. The goal of this study was to characterize the gene expression profile of a pure population of IVIg-generated induced Treg (iTreg). Treg were isolated from mice sensitized and challenged with ovalbumin (OVA). Animals were treated with IVIg to generate iTreg with or without DTx endogenous Treg pre-depletion. Human serum albumin (HSA) was used as a control protein treatment. RNA was isolated from 4 biological replicates for each condition. 12 samples in total were hybridized to Affymetrix gene expression microarrays. variable: treatment: OVA-HSA-OVA - DTx: BMR133, BMR134, BMR135, BMR136 variable: treatment: OVA-IVIg-OVA - DTx: BMR137, BMR138, BR139, BMR140 variable: treatment: OVA-IVIg-OVA + DTx: BMR141, BMR142, BMR143, BMR144 repeat: biological replicate: eTreg: BMR133, BMR134, BMR135, BMR136 repeat: biological replicate: e_iTreg: BMR137, BMR138, BR139, BMR140 repeat: biological replicate: IVIg-iTreg: BMR141, BMR142, BMR143, BMR144

2016-08-10 | E-GEOD-71811 | biostudies-arrayexpress

Liver gene expression profiles of Tribbles-homolog1 (Trib1)/Ldl-receptor (Ldlr) double-knockout mice against Ldlr-knockout mice

Project description:Genetic variants at the TRIB1 (Tribbles-homolog 1) gene locus are strongly associated with plasma lipid traits and the risk of coronary artery disease in humans. In this study, we analyzed the consequences of Trib1-deficiency (Trib1-/-) on hepatic gene expression in mice on the atherosclerosis-susceptible LDL-receptor deficient (Ldlr-/-) background. Therefore, Trib1-/- mice were crossed onto the Ldlr-/- background to generate double knockout mice (Trib1-/-Ldlr-/-) and fed a semisynthetic, modified AIN76 diet (0.02% cholesterol, 4.3% fat). At 20 weeks of age, Trib1-/-Ldlr-/- mice and Trib1+/+Ldlr-/- control mice were sacrificed and liver tissue was snap frozen in liquid nitrogen and stored at -80°C until further processing. Total RNA was isolated from liver tissue (n=8 mice per genotype) and subjected to microarray gene expression analysis.

2023-03-15 | E-MTAB-12669 | biostudies-arrayexpress

D2Ome - rate constant estimation from heavy water metabolic labeling and LC-MS

Project description:We developed a software for extraction of protein decay rate constants from heavy water labeling followed by LC-MS experiments. We illustrate the program on application to normal diet and western diet fed LDLR-/- mice. We demonstrate that proteins subunits of 40S ribosomal complex have reduced stability in western diet fet mice.

2019-04-10 | PXD009493 | Pride

Intermittent childhood hyperlipidaemia alters resident arterial macrophages and accelerates atherosclerosis

Project description:Peritoneal macrophages were obtained by intraperitoneal injection of cold PBS from Ldlr−/− mice reconstituted with Lyz2Cre+/− Nrp1flox/flox or Lyz2Cre+/− Nrp1+/+ bone marrow.

2024-07-30 | E-MTAB-12761 | biostudies-arrayexpress

STRUCTURE OF APOLIPOPROTEIN B100 BOUND TO LOW-DENSITY LIPOPROTEIN RECEPTOR

Project description:Apolipoprotein B100 (apoB100) is the main structural protein on Low-density Lipoproteins (LDL) and is the principal ligand for the LDL receptor (LDLR). Because the tertiary structure of apoB100 on LDL has not been determined, the detailed molecular interaction between apoB100 and LDLR is unknown. Here we present the structures of LDL bound to the LDLR and an apoB100 nanobody complex at 4.18Å resolution and a second LDL-LDLR interface at 4.83Å resolution.

2024-12-06 | PXD056510 | Pride

Attenuation of early atherogenesis in LDLR-KO mice by proteasome inhibition

Project description:To get further insights into the processes leading to attenuation of early stage atherosclerosis in the bortezomib treated LDLR-KO mice, microarray profiling of gene expression was performed on RNA taken from whole aortae of bortezomib treated LDLR-KO and sham- treated LDLR-KO mice fed a Western diet for 6 weeks and compared the changes in gene expression in both groups to non-atherosclerotic CD animals. Male 10-week-old LDLR-KO mice (B6.129S7-Ldlrtm1Her/J; JAX Mice, Boston) were fed a Western-type diet for 6 weeks ad libitum and received intraperitoneal injections of bortezomib (50 µg/kg body weight; WD+Bor) or saline (WD) twice weekly (n = 4). Mice that were fed normal diet served as chow diet control (CD; n = 4). Gene expression in aortic tissue was measured. Two independent experiments were performed.

2011-07-13 | E-GEOD-30638 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data