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Overexpression of cSki in hematopoietic stem and progenitor cells

ABSTRACT: Human cSki was overexpressed using MIGR1 retrovirus in sorted murine Lin-c-Kit+Sca-1+ cells. Cells were infected and cultured for 2 days after infection prior to isolation of GFP+ve cells and microarray. GFP+ve MIGR1 and cSKI cells were compared. Each sample represents an independent infection with either cSki or MIGR1 Comparison of GFP+ve LKS+ infected with MIGR1 and cSki

ORGANISM(S): Mus musculus

SUBMITTER: Carl Walkley

PROVIDER: E-GEOD-39457 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The SKI proto-oncogene enhances the in vivo repopulation of hematopoietic stem cells and causes myeloproliferative disease.

Singbrant Sofie S Wall Meaghan M Moody Jennifer J Karlsson Göran G Chalk Alistair M AM Liddicoat Brian B Russell Megan R MR Walkley Carl R CR Karlsson Stefan S

Haematologica 20140110 4

The proto-oncogene SKI is highly expressed in human myeloid leukemia and also in murine hematopoietic stem cells. However, its operative relevance in these cells remains elusive. We have over-expressed SKI to define its intrinsic role in hematopoiesis and myeloid neoplasms, which resulted in a robust competitive advantage upon transplantation, a complete dominance of the stem and progenitor compartments, and a marked enhancement of myeloid differentiation at the expense of other lineages. Accord ...[more]

PMID: 24415629

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