Identification of microarray probe signals constantly present in multiple sample types
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Stephen Krawetz
PROVIDER: E-GEOD-39528 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Identification of artifactual microarray probe signals constantly present in multiple sample types.
Mao Shihong S Souza Aletheia Lima AL Goodrich Robert J RJ Krawetz Stephen A SA
BioTechniques 20120801 2
The detection, identification, and quantitation of transcripts have evolved from simple Northern analysis, cDNA cloning, and sequencing to RT-PCR, microarrays, and now digital gene expression using ultra-high-throughput RNA sequencing (RNA-Seq). During the course of our studies we observed that some microarray probes show very high signal intensity values yet are discordant when compared with RNA-Seq. A total of 99 probes from approximately 30,000 were identified as consistently discordant in fo ...[more]
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