Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of polysomal mRNA populations of mouse oocytes and zygotes

ABSTRACT: Transcriptional activation in mammalian embryos occurs in a stepwise manner. In mice, it begins at the late one-cell stage, followed by a minor wave of activation at the early two-cell stage, and then the major genome activation (MGA) at the late two-cell stage. Cellular homeostasis, metabolism, cell cycle, and developmental events are orchestrated before MGA by time-dependent changes in the array of maternal transcripts being translated (i.e., the translatome). Despite the importance of maternal mRNA and its correct recruitment for development, neither the array of recruited mRNA nor the regulatory mechanisms operating have been well cheracterized. We present the first comprehensive analysis of changes in the maternal component of the zygotic translatome during the transition from oocyte to late one-cell stage embryo, revealing global transitions in the functional classes of translated maternal mRNAs, and apparent changes in the underlying cis-regulatory mechanisms. Batches of 160-500 MII oocytes or one-cell embryos. 4 independent replicates per sample kind. Lysed and processed for polysomal mRNA extraction. Two rounds of IVT.

ORGANISM(S): Mus musculus

SUBMITTER: Keith Latham

PROVIDER: E-GEOD-3962 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Analysis of polysomal mRNA populations of mouse oocytes and zygotes: dynamic changes in maternal mRNA utilization and function.

Potireddy Santhi S Vassena Rita R Patel Bela G BG Latham Keith E KE

Developmental biology 20060617 1

Transcriptional activation in mammalian embryos occurs in a stepwise manner. In mice, it begins at the late one-cell stage, followed by a minor wave of activation at the early two-cell stage, and then the major genome activation event (MGA) at the late two-cell stage. Cellular homeostasis, metabolism, cell cycle, and developmental events are orchestrated before MGA by time-dependent changes in the array of maternal transcripts being translated. Many elegant studies have documented the importance ...[more]

PMID: 16860309

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Project description:Hematopoietic stem cells (HSCs) play a crucial role in maintaining the steady supply of blood cells throughout an organism’s lifetime. Understanding the regulatory mechanisms governing HSC homeostasis is of particular importance for regenerative medicine and stem cell-based therapies. Translatome refers to the ensemble mRNA transcripts being actively translated on ribosomes within a cell type, under specific physio/pathological conditions. Translatome profiling provides insights into the regulation of gene expression at the translational level, going beyond traditional transcriptome analysis. In this study, we systematically profiled the translatome landscapes of hematopoietic cells, and unraveled novel insights on HSC functionality and regulation mechanisms. Moreover, for further exploring underlying regulation mechanisms of translatome, we systematically screened the snoRNAs in hematopoietic cells and uncovered an indispensable role for the maternally expressed snoRNA cluster SNORD113-114 at the Dlk1-Gtl2 imprinting region in regulation of HSC self-renewal. Maternal knockout of this cluster (Mat-KO) significantly impaired HSC self-renewal, while loss of the paternal allele caused no obvious abnormal hematological phenotype. Mechanistically, Mat-KO resulted in dysregulation of translation machinery, in terms of 2’-O-Me modifications on rRNAs, rRNA processing, ribosome function and translatome. Finally, Loss of SNORD113-114 cluster induced impaired ribosome biogenesis checkpoint/nucleolar stress in HSCs, which exempted p53 from proteasomal degradation and eventually led to apoptosis in HSCs. Collectively, our findings shed light on the intricate molecular mechanisms governing HSC behavior and provide potential therapeutic targets for enhancing HSC function and advancing regenerative medicine. Understanding the translation regulation of gene expression in HSCs has significant implications for stem cell biology and offers promising avenues for clinical applications.

Dataset Information

Transcription profiling of polysomal mRNA populations of mouse oocytes and zygotes

Publications

Analysis of polysomal mRNA populations of mouse oocytes and zygotes: dynamic changes in maternal mRNA utilization and function.

Similar Datasets

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