Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Functional characterization of the RNA chaperone Hfq in opportunistic human pathogen Stenotrophomonas maltophilia

ABSTRACT: Stenotrophomonas maltophilia is an important opportunistic pathogen affecting primarily hospitalized and immuno-compromised hosts. We constructed an hfq deletion mutant (Delta-hfq) of S. maltophilia, and compared the behaviour of wild-type and Delta-hfq S. maltophilia cells in a variety of assays. Differential RNA sequencing analysis (dRNA-seq) of RNA isolated from S. maltophilia wild-type and Delta-hfq strains showed that Hfq regulates expression of genes encoding flagellar and fimbrial components, transmembrane proteins, as well as enzymes involved in different metabolic pathways. Moreover, we analysed expression of several sRNAs identified by dRNA-seq in wild-type. The accumulation of two sRNAs was strongly reduced in the absence of Hfq. TEX (terminator exonuclease) treated and untreated libraries of the wild type and the Delta-hfq mutant were sequenced and compared

ORGANISM(S): Stenotrophomonas maltophilia

SUBMITTER: Konrad FM-CM-6rstner 

PROVIDER: E-GEOD-39705 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Functional characterization of the RNA chaperone Hfq in the opportunistic human pathogen Stenotrophomonas maltophilia.

Roscetto Emanuela E   Angrisano Tiziana T   Costa Valerio V   Casalino Mariassunta M   Förstner Konrad U KU   Sharma Cynthia M CM   Di Nocera Pier Paolo PP   De Gregorio Eliana E  

Journal of bacteriology 20120824 21

Hfq is an RNA-binding protein known to regulate a variety of cellular processes by interacting with small RNAs (sRNAs) and mRNAs in prokaryotes. Stenotrophomonas maltophilia is an important opportunistic pathogen affecting primarily hospitalized and immunocompromised hosts. We constructed an hfq deletion mutant (Δhfq) of S. maltophilia and compared the behaviors of wild-type and Δhfq S. maltophilia cells in a variety of assays. This revealed that S. maltophilia Hfq plays a role in biofilm format  ...[more]

Similar Datasets

Transcriptomics Functional characterization of the RNA chaperone Hfq in opportunistic human pathogen Stenotrophomonas maltophilia
2012-10-02 | GSE39705 | GEO
Transcriptomics Salmonella enterica serovar Typhimurium 4/74 transcriptomics using strand-specific RNA-seq
2013-12-18 | E-GEOD-49829 | biostudies-arrayexpress
Transcriptomics Identifying genes of Escherichia coli involved in interactions with Stenotrophomonas maltophilia in planktonic cultures and biofilms using transcriptomic analysis
2013-12-30 | E-GEOD-24915 | biostudies-arrayexpress
Transcriptomics dRNA-seq of Shewanella oneidensis MR-1
2014-06-12 | E-GEOD-58292 | biostudies-arrayexpress
Transcriptomics Identification of Hfq bound RNA by co-immunoprecipitation and RNA-sequencing
2014-12-04 | E-GEOD-59123 | biostudies-arrayexpress
Transcriptomics RNA sequencing based analysis of the bacterial transcriptome
2014-12-11 | E-GEOD-58556 | biostudies-arrayexpress
Transcriptomics Escherichia coli planktonic cultures: mono-species culture vs. mixed-species culture
2013-12-30 | E-GEOD-24913 | biostudies-arrayexpress
Transcriptomics Escherichia coli biofilm: mono-species culture vs. mixed-species culture
2013-12-30 | E-GEOD-24912 | biostudies-arrayexpress
Transcriptomics Unexpected complexity of the Escherichia coli K-12 transcriptome architecture revealed by single nucleotide resolution RNA sequencing
2014-07-08 | E-GEOD-52059 | biostudies-arrayexpress
Transcriptomics Differential RNA-Seq (dRNA-seq) data from Sphingopyxis granuli strain TFA grown in two different carbon sources and RNA-seq from Hfq-coIP experiment.
2018-08-14 | GSE111181 | GEO