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Purified human leukocyte subtype DNA methylation profiles

ABSTRACT: The objective of the study was to identify differentially methylated regions of DNA (DMRs) that distinguish human leukocyte subtypes, and hence serve as biomarkers for those immune cell types. This file contains Illumina Infinium HumanMethylation27 BeadChip data for human leukocyte subtypes that were purified from whole blood samples via magnetic activated cell sorting (MACS) and purity confirmed by flourescence activated cell sorting (FACS). Bisulphite converted DNA from the 73 samples were hybridised to the Illumina Infinium 27k Human Methylation Beadchip v1.2

ORGANISM(S): Homo sapiens

SUBMITTER: William Accomando 

PROVIDER: E-GEOD-39981 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Decreased NK cells in patients with head and neck cancer determined in archival DNA.

Accomando William P WP   Wiencke John K JK   Houseman E Andres EA   Butler Rondi A RA   Zheng Shichun S   Nelson Heather H HH   Kelsey Karl T KT  

Clinical cancer research : an official journal of the American Association for Cancer Research 20120926 22

<h4>Purpose</h4>Natural killer (NK) cells are a key element of the innate immune system implicated in human cancer. To examine NK cell levels in archived bloods from a study of human head and neck squamous cell carcinoma (HNSCC), a new DNA-based quantification method was developed.<h4>Experimental design</h4>NK cell-specific DNA methylation was identified by analyzing DNA methylation and mRNA array data from purified blood leukocyte subtypes (NK, T, B, monocytes, granulocytes), and confirmed via  ...[more]

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