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RNASeq analysis of Salmonella B cell infection model

ABSTRACT: We report the transcriptome analysis of S. enterica 14028 during infection of murine B cells. Mice spleens were infected with Salmonella enterica 14028. Mouse spleens were harvested and cells FACS sorted into various immune cells containing bacteria. Short reads were mapped to the S. enterica 14028 genome. Mice were infected with S. enterica strain 14028. Spleens were collected, homogenized and cells FACS sorted. Total RNA was extracted using the Ambion mirVana RNA isolation kit. Purified RNA was depleted of rRNA utilizing the epicentre ribozero metabacteria and eukaryotic kits.

ORGANISM(S): Mus musculus

SUBMITTER: Marcus Jones

PROVIDER: E-GEOD-40211 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNASeq analysis of Salmonella B cell infection model

Project description:We report the transcriptome analysis of S. enterica 14028 during infection of murine B cells. Mice spleens were infected with Salmonella enterica 14028. Mouse spleens were harvested and cells FACS sorted into various immune cells containing bacteria. Short reads were mapped to the S. enterica 14028 genome.

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