Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome profiling reveals a genomewide role of G-proteins


ABSTRACT: Knock out mutants of GPA1 (gpa1-5), GCR1 (gcr1-5) as well as their double mutant (gpa1-5 gcr1-5) were used to obtain genomewide transcriptome profile to deduce genomewide effect of both the subunits alone or in combination. Heterotrimeric G-protein signaling pathways have been implicated in a variety of biological processes in plants. While an impressive range of important plant responses are known to be mediated by G-protein signaling pathways, the genes that respond through these pathways are not yet known completely. More importantly, the role of G-proteins in mediating plant metabolic regulation, which is well established in animal systems, has not received adequate attention. The availability of loss-of-function G_alpha (GPA1) and G-protein coupled receptor (GCR1) mutant(s) as well as their double mutant in Arabidopsis provided an opportunity for a genome-wide microarray analysis in the current study for the first time. transcriptome profile of mutants gpa1-5, gcr1-5 and double mutant gpa1-5 gcr1-5 were compared to that of the corresponding wild type, ws2. biological duplicates of each sample wa used and hybridized using single color array

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Nandula Raghuram 

PROVIDER: E-GEOD-40217 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptome analysis of Arabidopsis GCR1 mutant reveals its roles in stress, hormones, secondary metabolism and phosphate starvation.

Chakraborty Navjyoti N   Sharma Priyanka P   Kanyuka Kostya K   Pathak Ravi R RR   Choudhury Devapriya D   Hooley Richard A RA   Raghuram Nandula N  

PloS one 20150210 2


The controversy over the existence or the need for G-protein coupled receptors (GPCRs) in plant G-protein signalling has overshadowed a more fundamental quest for the role of AtGCR1, the most studied and often considered the best candidate for GPCR in plants. Our whole transcriptome microarray analysis of the GCR1-knock-out mutant (gcr1-5) in Arabidopsis thaliana revealed 350 differentially expressed genes spanning all chromosomes. Many of them were hitherto unknown in the context of GCR1 or G-p  ...[more]

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