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Milbemycins: more than efflux inhibitors for fungal pathogens

ABSTRACT: This study investigated the effect of milbemycons as efflux inhibitors and antifungal agents. Milbemycin oxims can inhibit growth of Candida glabrata and C. albicans. The effect of milbemycins on transcriptomes was inbvestigated. Gene expression was measured in C. glabrata milbemycin-treated cells (1h) and compared to untreated cells in the incubation mediun RPMI. The one-color system was used. Four biological replicates were used for each condition. Gene expression was measured in C. albicans milbemycin-treated cells (1h) and compared to untreated cells in the incubation mediun RPMI. The one-color system was used. Three biological replicates were used for each condition.

ORGANISM(S): Candida albicans

SUBMITTER: Dominique Sanglard

PROVIDER: E-GEOD-40232 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Milbemycins: more than efflux inhibitors for fungal pathogens.

Silva Luis Vale LV Sanguinetti Maurizio M Vandeputte Patrick P Torelli Riccardo R Rochat Bertrand B Sanglard Dominique D

Antimicrobial agents and chemotherapy 20121203 2

Existing antifungal agents are still confronted to activities limited to specific fungal species and to the development of resistance. Several improvements are possible either by tackling and overcoming resistance or exacerbating the activity of existing antifungal agents. In Candida glabrata, azole resistance is almost exclusively mediated by ABC transporters (including C. glabrata CDR1 [CgCDR1] and CgCDR2) via gain-of-function mutations in the transcriptional activator CgPDR1 or by mitochondri ...[more]

PMID: 23208712

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