Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Analysis of gene content among Group II Clostridium botulinum strains

ABSTRACT: The purpose of this study was to compare the gene content of several group II C. botulinum strains to determine the utility of DNA microarrays for subtyping this organism. Group II type B, E, and F C. botulinum strains were compared in a study to examine the gene content of a unique type E strain (CDC66177). One replicate of genomic DNA isolated from 16 type E strains, 4 type B strains, and 2 type F strains were examined.

ORGANISM(S): Clostridium botulinum

SUBMITTER: Brian Raphael

PROVIDER: E-GEOD-40271 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Analysis of a unique Clostridium botulinum strain from the Southern hemisphere producing a novel type E botulinum neurotoxin subtype.

Raphael Brian H BH Lautenschlager Matthew M Kalb Suzanne R SR de Jong Laura I T LI Frace Michael M Lúquez Carolina C Barr John R JR Fernández Rafael A RA Maslanka Susan E SE

BMC microbiology 20121031

<h4>Background</h4>Clostridium botulinum strains that produce botulinum neurotoxin type E (BoNT/E) are most commonly isolated from botulism cases, marine environments, and animals in regions of high latitude in the Northern hemisphere. A strain of C. botulinum type E (CDC66177) was isolated from soil in Chubut, Argentina. Previous studies showed that the amino acid sequences of BoNT/E produced by various strains differ by < 6% and that the type E neurotoxin gene cluster inserts into the rarA ope ...[more]

PMID: 23113872

Similar Datasets

Genomic content analysis of Clostridium botulinum type F strains

Project description:The purpose of this study was to determine the level of genomic content similarity among selected strains of Clostridium botuinum type F strains. In this study, strains were selected that had already been chacaterized by botulinum neurotoxin gene sequencing. Strains harboring bont/F1, bont/F4 and bont/F5 were compared.

2013-12-19 | E-GEOD-53456 | biostudies-arrayexpress

Detection and differentiation of Clostridium botulinum strains

Project description:Strains were differentiated on the basis of hybridization to probes representing strain variable regions in C. botulinum strain ATCC 3502. Probes for selected genes (eg. toxin genes) were also featured on the microarray to allow detection of other serotypes/subtypes. 44 strains were evaluated for selected gene detection and/or strain differentiation. DNA from strain ATCC 3502 was used as a control as the featured probes were based on the ATCC 3502 genome sequence.

2010-05-18 | E-GEOD-16101 | biostudies-arrayexpress

Focused DNA microarray to differentiate Clostridium botulinum type A strains

Project description:Strains were differentiated on the basis of hybridization to probes representing strain variable regions in C. botulinum strain ATCC 3502. Probes for selected genes (eg. toxin genes) were also featured on the microarray to allow detection of other serotypes/subtypes. 27 strains were evaluated for selected gene detection and/or strain differentiation. DNA from strain ATCC 3502 was used as a control as the featured probes were based on the ATCC 3502 genome sequence.

2010-05-05 | E-GEOD-18086 | biostudies-arrayexpress

Comparision of Clostridium botulinum type A (HA-/ORFX+ A1) isolates

Project description:The purpose of this study was to evaluate differences in gene content between tests samples isolated from separate food sources. A single biological sample was used for each test strain examined

2013-09-26 | E-GEOD-51155 | biostudies-arrayexpress

Analysis of gene content among Group II Clostridium botulinum strains

Project description:The purpose of this study was to compare the gene content of several group II C. botulinum strains to determine the utility of DNA microarrays for subtyping this organism. Group II type B, E, and F C. botulinum strains were compared in a study to examine the gene content of a unique type E strain (CDC66177).

2012-11-08 | GSE40271 | GEO

Use of CGH microarrays to identify strain variable genomic regions in C. botulinum ATCC 3502

Project description:Comparative genomic hybridization microarrays featuring overlapping probes spanning the entire C. botulinum type A1 strain ATCC 3502 genome were used to identify regions whose presence are variable among a diverse panel of type A C. botulinum strains. For each hybridization, the reference strain (C. botulinum ATCC 3502) was labeled with Cy5 and test strains were labeled with Cy3.

2010-05-19 | E-GEOD-16010 | biostudies-arrayexpress

Clostridium botulinum

Project description:Genomic content analysis of Clostridium botulinum type F strains

| PRJNA232050 | ENA

Use of CGH microarrays to identify strain variable genomic regions in C. botulinum ATCC 3502

2009-05-15 | GSE16010 | GEO

Comparative genomic hybridization of Clostridium sporogenes and Clostridium botulinum strains

Project description:Clostridium botulinum is a heterogeneous Gram-positive species that comprises four genetically and physiologically distinct groups of bacteria that share the ability to produce botulinum neurotoxin, the most poisonous toxin known to man, and the causative agent of botulism, a severe disease of humans and animals. We report here the complete genome sequence of a representative of Group I (proteolytic) C. botulinum (strain Hall A, ATCC 3502). The genome consists of a chromosome (3,886,916 bp) and a plasmid (16,344 bp) which carry 3,650 and 19 predicted genes, respectively. Consistent with the proteolytic phenotype of this strain, the genome harbours a large number of genes encoding secreted proteases and enzymes involved in uptake and metabolism of amino acids. The genome also reveals a hitherto unknown ability of C. botulinum to degrade chitin. There is a significant lack of recently acquired DNA, indicating a stable genomic content, in strong contrast to the fluid genome of C. difficile, which can form longer-term relationships with its host. Overall, the genome indicates that C. botulinum is adapted to a saprophytic lifestyle both in soil and aquatic environments. This pathogen relies on its toxin to rapidly kill a wide range of prey species, and to gain access to nutrient sources, it releases a large number of extracellular enzymes to soften and destroy rotting or decayed tissues.

2007-05-23 | E-TABM-264 | biostudies-arrayexpress

Co-hybridization of Adenovirus type 40 and 41

Project description:Co-hybridization of Adenovirus type 40 and 41 to test probe specificity for closely related viral targets. ATCC strains of Adenovirus type 40 and 41 were hybridized to the array as a control run and a proof of concept. Degree of cross hybridization between polio nucleic acid and non-adenovirus 40 and 41 probes was evaluated. Specificity of the probe design was determined between closely related members of the same virus family. Keywords: control study: target detection and specificity 2 lab strains of adenovirus (type 40 and 41) were extracted directly from ATCC samples. Viral DNA was was extracted and labeled with Cy3 and Cy5 dyes for Adenovirus type 40 and 41 respectively.

2010-05-26 | E-GEOD-10569 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data