Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Young Kim
PROVIDER: E-GEOD-4079 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Bredel Markus M Bredel Claudia C Juric Dejan D Kim Young Y Vogel Hannes H Harsh Griffith R GR Recht Lawrence D LD Pollack Jonathan R JR Sikic Branimir I BI
The Journal of molecular diagnostics : JMD 20050501 2
Sufficient quantity of genomic DNA can be a bottleneck in genome-wide analysis of clinical tissue samples. DNA polymerase Phi29 can be used for the random-primed amplification of whole genomes, although the amplification may introduce bias in gene dosage. We have performed a detailed investigation of this technique in archival fresh-frozen and formalin-fixed/paraffin-embedded tumor DNA by using cDNA microarray-based comparative genomic hybridization. Phi29 amplified DNA from matched pairs of fre ...[more]