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SM020 - Infection with SARS MA15 of C57BL/6J mice and KEPI (ppp1r14c) knockouts

ABSTRACT: Comparing the host response of C57BL/6J mice to KEPI (ppp1r14c) knockouts in the same strain of mice. Groups of 10-week-old C57BL/6 mice or KEPI (ppp1r14c) knockout mice were infected with SARS MA15 at a dose of 10^5 PFU or mock infected. Mice were euthanized on days 4 and 7 post-infection to measure virus load and isolate samples for measurement of virus load, lung pathology, transcriptional analysis and proteomics analysis. Mock-infected animals were also harvested at each time point. Mice were weighed every 24 hours to measure general disease progression and any mouse approaching 30% weight loss was euthanized.

ORGANISM(S): Mus musculus

SUBMITTER: Richard Green

PROVIDER: E-GEOD-40827 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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