Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

N2 worms treated with Resveratrol


ABSTRACT: Eggs were isolated by hypochlorite treatment and allowed to hatch in the absence of food as L1 stage arrested worms in S-buffer. Worms were then transferred to large plates containing NGM alone or NGM+ resveratrol and allowed to grow till young adult stage (ca 40 hours). Worms were harvested and total RNA extracted using Trizol reagent. Poly-A+ RNA was isolated using Qiagen midi-prep kit and used for microarray hybridization. A compound treatment design type is where the response to administration of a compound or chemical (including biological compounds such as hormones) is assayed. Compound Based Treatment: Resveratrol Computed

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Mohan Viswanathan 

PROVIDER: E-GEOD-4094 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

A role for SIR-2.1 regulation of ER stress response genes in determining C. elegans life span.

Viswanathan Mohan M   Kim Stuart K SK   Berdichevsky Ala A   Guarente Leonard L  

Developmental cell 20051101 5


C. elegans SIR-2.1, a member of the Sir-2 family of NAD(+)-dependent protein deacetylases, has been shown to regulate nematode aging via the insulin/IGF pathway transcription factor daf-16. Treatment of C. elegans with the small molecule resveratrol, however, extends life span in a manner fully dependent upon sir-2.1, but independent of daf-16. Microarray analysis of worms treated with resveratrol demonstrates the transcriptional induction of a family of genes encoding prion-like glutamine/aspar  ...[more]

Similar Datasets

2010-06-24 | E-GEOD-15361 | biostudies-arrayexpress
2006-01-06 | E-GEOD-3988 | biostudies-arrayexpress
2006-01-27 | E-GEOD-4111 | biostudies-arrayexpress
2010-06-24 | E-GEOD-15297 | biostudies-arrayexpress
2008-02-07 | E-GEOD-7947 | biostudies-arrayexpress
2010-05-12 | E-GEOD-18151 | biostudies-arrayexpress
2007-09-13 | E-GEOD-9027 | biostudies-arrayexpress
2010-06-24 | E-GEOD-16103 | biostudies-arrayexpress
2010-06-24 | E-GEOD-16107 | biostudies-arrayexpress
2005-08-11 | E-GEOD-3107 | biostudies-arrayexpress