Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

35 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Competition between viral-derived and endogenous small RNA pathways regulates gene expression in response to viral infection in C.elegans.

ABSTRACT: This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

ORGANISM(S): Caenorhabditis elegans

SUBMITTER: Eric Miska

PROVIDER: E-GEOD-41058 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Analysis of gene expression changes upon infection of C.elegans with Orsay virus

Project description:Analysis of the transcriptional response to viral infection in C.elegans. Comparison of infected and uninfected animals for three strains: N2 (resistant), JU1580 (sensitive), RDE-1 (sensitive)

2013-06-01 | E-GEOD-41056 | biostudies-arrayexpress

Analysis of small RNA response to viral infection in C.elegans

Project description:Dissection of the small RNA pathway required for generation of an antiviral small RNA response Small non-coding RNA (~15-30nt) was extracted from animals infected with the Orsay virus

2013-06-01 | E-GEOD-41693 | biostudies-arrayexpress

Changes in small RNAs upon Viral infection of C.elegans

Project description:Attempt to identify small non-coding RNAs that change in levels as a result of viral infection of C.elegans Small non-coding RNA (18-30nt) was extracted from animals either infected with Orsay virus or uninfected as indicated.

2013-06-01 | E-GEOD-41057 | biostudies-arrayexpress

Function, targets and evolution of Caenorhabditis elegans piRNAs

Project description:This SuperSeries is composed of the following subset Series: GSE28617: Function, targets and evolution of Caenorhabditis elegans piRNAs (small RNA-Seq) GSE37432: Function, targets and evolution of Caenorhabditis elegans piRNAs (mRNA) Refer to individual Series

2012-06-17 | E-GEOD-37433 | biostudies-arrayexpress

Function, targets and evolution of Caenorhabditis elegans piRNAs (small RNA-Seq)

Project description:piRNAs are required to maintain germline integrity and fertility but their mechanism of action is poorly understood. Here we demonstrate that C. elegans piRNAs silence transcripts in trans through imperfectly complementary sites. We find that target silencing is independent of Piwi endonuclease activity or “slicing”. Instead, we show that piRNAs initiate a localized secondary endogenous small interfering RNA (endo-siRNA) response. Endogenous protein-coding gene, pseudogene and transposon transcripts exhibit Piwi-dependent endo-siRNAs at sites complementary to piRNAs and are derepressed in Piwi mutants. Genomic loci of piRNA biogenesis are depleted of protein-coding genes but not pseudogenes or transposons. Our data suggest that nematode piRNA clusters are evolving to generate piRNAs against active mobile elements. Thus, piRNAs provide heritable, sequence-specific triggers for RNAi in C. elegans. 7 small RNA libraries were sequenced as part of 25 flow cell lanes on the Illumina GA II platform. Samples were treated with tobacco acid pyrophosphatase to allow cloning of small RNAs with a 5'-triphosphate. Samples were labelled for multiplexing using 4-bp 5'-barcodes or barcodes included in Illumina TruSeq adapters. In most cases a single flow cell lane included several multiplexed libraries.

2012-06-18 | E-GEOD-28617 | biostudies-arrayexpress

Transgenerational profiling of small non-coding RNAs in C.elegans RSD mutants

Project description:Attempt to identify progressive changes in small non-coding RNA levels in C.elegans lacking RSD-2 or RSD-6. Small non-coding RNA (18-30nt) was extracted from animals the indicated number of generations after homozygosity was established

2013-06-01 | E-GEOD-40457 | biostudies-arrayexpress

Transgenerational changes in small RNA profiles of C.elegans mutants lacking PRG-1

Project description:An attempt to identify small non-coding RNAs that change with increasing generations after becoming homozygous for the loss of PRG-1 Small non-coding RNA (18-30nt) was extracted from animals the indicated number of generations after homozygosity was established

2013-06-01 | E-GEOD-40572 | biostudies-arrayexpress

RSD-2 and RSD-6 promote germ cell immortality by repressing spermatogenesis and repeat loci via small interfering RNAs

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-06-01 | E-GEOD-40460 | biostudies-arrayexpress

Reduced insulin/IGF-1-like signaling restores germ cell immortality in Caenorhabditis elegans Piwi mutants

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-06-01 | E-GEOD-40573 | biostudies-arrayexpress

Endogenous DAF-16 genome-wide recruitment under low Insulin signaling condition in Caenorhabditis elegans

Project description:In order to understand the complexity of gene regulation downstream of IIS, we used anti-DAF-16 antibody, we report the first genome-wide ChIP-sequencing study of endogenous DAF-16 recruitment in daf-2(e1370). We also report the average bin-wise normalized read count of ZFP-1 and DAF-16 on DAF-16 summits ChIP-Seq profile of DAF-16 in mix stage

2016-03-01 | E-GEOD-63865 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data