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Heterobasidion annosum gene expression from mycelium grown on different substrates

ABSTRACT: Transcript profiles of H. annosum from mycelium grown on different substrates and under different stresses were analyzed. The array probes were designed from gene models taken from the Joint Genome Institute (JGI, department of energy) H. irregulare genome sequence version 1. One aim of this study was to compare gene expression profiles of H. annosum pre-grown on secondary metabolites from the biocontrol agent Phlebiopsis gigantea. We performed six hybridizations with samples derived from H. annosum grown in either liquid Malt Extract Medium (three biological replicates) or on secondary metabolite from Phlebiopsis gigantea (three biological replicates). The Heterobasidion irregulare custom-exon expression array (4 x 72K) manufactured by Roche NimbleGen Systems Limited (Madison, WI) (http://www.nimblegen.com/products/exp/index.html) contained five independent, non-identical, 60-mer probes per gene model coding sequence. For 12,199 of the 12,299 annotated protein-coding gene models, probes could be designed. For 19 gene models, no probes could be generated, and 81 gene models shared all five probes with other gene models. Included in the array were 916 random 60-mer control probes and labelling controls. For 2032 randomly chosen gene models, technical duplicates were included on the array.

ORGANISM(S): Heterobasidion annosum

SUBMITTER: Annegret Kohler

PROVIDER: E-GEOD-41301 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Heterobasidion annosum gene expression from different tissues and mycelium grown on different substrates and under different biotic and abiotic stresses

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