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Transcription profiling of S. cerevisiae wild type and amino-terminal mutant histone H3 and H4 strains

ABSTRACT: Yeast lacking the H3 or H4 amino termini, and corresponding wild type strains, were grown in synthetic media. These conditions induce Gcn4-activated transcription. Experiment Overall Design: Three replicates of the H4del(2-26) and corresponding wild type strains, and four replicates of the H3del(1-28) and corresponding wild type strains, were performed. Note the strains are congenic, but differ in whether the histones were present on TRP1 marked (H4) or LEU2 marked (H3) plasmids. Samples were analysed using Affymetrix S98 microarrays.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Randall Heywood Morse

PROVIDER: E-GEOD-4135 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Contribution of the histone H3 and H4 amino termini to Gcn4p- and Gcn5p-mediated transcription in yeast.

Yu Cailin C Palumbo Michael J MJ Lawrence Charles E CE Morse Randall H RH

The Journal of biological chemistry 20060204 14

Histone amino termini are post-translationally modified by both transcriptional coactivators and corepressors, but the extent to which the relevant histone modifications contribute to gene expression, and the mechanisms by which they do so, are incompletely understood. To address this issue, we have examined the contributions of the histone H3 and H4 amino termini, and of the coactivator and histone acetyltransferase Gcn5p, to activation of a small group of Gcn4p-activated genes. The histone H3 ...[more]

PMID: 16461773

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