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Genome-wide nucleosome position maps in Saccharomyces cerevisiae

ABSTRACT: Paired-end sequencing study of nucleosomal DNA prepared from budding yeast by micrococcal nuclease digestion. Comparison of control cells with cells treated with 10 mM 3-aminotriazole for 20 minutes

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: David Clark

PROVIDER: E-GEOD-26493 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The centromeric nucleosome of budding yeast is perfectly positioned and covers the entire centromere.

Cole Hope A HA Howard Bruce H BH Clark David J DJ

Proceedings of the National Academy of Sciences of the United States of America 20110718 31

The centromeres of budding yeast are ~120 bp in size and contain three functional elements: an AT-rich region flanked by binding sites for Cbf1 and CBF3. A specialized nucleosome containing the H3 variant Cse4 (CenH3) is formed at the centromere. Our genome-wide paired-end sequencing of nucleosomal DNA reveals that the centromeric nucleosome contains a micrococcal nuclease-resistant kernel of 123-135 bp, depending on the centromere, and is therefore significantly shorter than the canonical nucle ...[more]

PMID: 21768332

Publication: 1/2

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