Transcriptome comparison of rokA mutant to wild-type in the Streptococcus pneumoniae D39.

ABSTRACT: The Gram-positive human pathogen Streptococcus pneumoniae possesses an unusually high number of gene clusters specific for carbohydrate utilization. This provides it with the ability to use a wide array of sugars, which may aid during infection and survival in different environmental conditions present in the host. In this study, the regulatory mechanism of transcription of a gene cluster, SPD0424-28, putatively encoding a cellobiose/lactose-specific PTS, is investigated. Upstream of SPD0424 a gene was identified encoding a ROK-family transcriptional regulator (RokA: SPD0423). DNA microarray and transcriptional reporter analyses with a rokA mutant revealed that RokA acts as a transcriptional repressor of the SPD0424-28 operon. In conclusion, we have characterized the role of RokA on gene e

ORGANISM(S): Streptococcus pneumoniae D39

SUBMITTER: Sulman Shafeeq 

PROVIDER: E-GEOD-41448 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress