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Transcriptome comparison of rokA mutant to wild-type in the Streptococcus pneumoniae D39.

ABSTRACT: The Gram-positive human pathogen Streptococcus pneumoniae possesses an unusually high number of gene clusters specific for carbohydrate utilization. This provides it with the ability to use a wide array of sugars, which may aid during infection and survival in different environmental conditions present in the host. In this study, the regulatory mechanism of transcription of a gene cluster, SPD0424-28, putatively encoding a cellobiose/lactose-specific PTS, is investigated. Upstream of SPD0424 a gene was identified encoding a ROK-family transcriptional regulator (RokA: SPD0423). DNA microarray and transcriptional reporter analyses with a rokA mutant revealed that RokA acts as a transcriptional repressor of the SPD0424-28 operon. In conclusion, we have characterized the role of RokA on gene expression of S. pneumoniae. One condition design comparision of two strains including a dye swap

ORGANISM(S): Streptococcus pneumoniae D39

SUBMITTER: Sulman Shafeeq

PROVIDER: E-GEOD-41448 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Characterization of the ROK-family transcriptional regulator RokA of Streptococcus pneumoniae D39.

Shafeeq Sulman S Kloosterman Tomas G TG Rajendran Vijayanand V Kuipers Oscar P OP

Microbiology (Reading, England) 20121018 Pt 12

The Gram-positive human pathogen Streptococcus pneumoniae possesses an unusually high number of gene clusters specific for carbohydrate utilization. This provides it with the ability to use a wide array of sugars, which may aid during infection and survival in different environmental conditions present in the host. In this study, the regulatory mechanism of transcription of a gene cluster, SPD0424-8, putatively encoding a cellobiose/lactose-specific phosphotransferase system is investigated. We ...[more]

PMID: 23082033

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