Comparison of the Streptococcus pneumoniae D39 wild-type to the D39 galR mutant [GM17]

ABSTRACT: We explored the regulatory mechanism of Leloir pathway genes in Streptococcus pneumoniae D39. Here, we demonstrate that the expression of galKT is galactose dependent. By microarray analysis and quantitative RT-PCR, we further show the role of the transcriptional regulator GalR, present upstream of galKT, as a transcriptional activator of galKT in the presence of galactose. Moreover, we predict a 19-bp regulatory site (5'-GATAGTTTAGTAAAATTTT-3' ) for the transcriptional regulator GalR in the promoter region of galK, which is also highly conserved in other streptococci. Growth comparison of Δ galK with the D39 wildtype strain grown in the presence of galactose shows that galK is required for the proper growth of S. pneumoniae on galactose. Comparison of the Streptococcus pneumoniae D39 galR mutant compared to D39 wild type in M17 medium+ 0.5% (w/v) glucose (GM17). One condition design comparison of two strains including a dye swap.

ORGANISM(S): Streptococcus pneumoniae D39

SUBMITTER: Sulman Shafeeq 

PROVIDER: E-GEOD-74500 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress