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Gene expression data in Meg-01 cells following transfection with platelet-enriched microRNA-1225-3p

ABSTRACT: Platelets from sickle cell disease patients have differentially expressed microRNAs as compared to platelets from healthy control subjects. The objective of this study is to overexpress an upregulated miRNA,miR-1225-3p, in MEG01 cells to identify putative targets. MEG-01 cells were transfected with microRNA-1225-3p mimic for overexpression. Simultaneously, a negative control (scrambled) RNA was transfected for comparison. Gene expression was measured at 24 hours after transfection. Five independent experiments were performed at the same time in each group: microRNA-1225-3p transfected and scrambled.

ORGANISM(S): Homo sapiens

SUBMITTER: Shilpa Jain

PROVIDER: E-GEOD-41573 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression of regulatory platelet microRNAs in patients with sickle cell disease.

Jain Shilpa S Kapetanaki Maria G MG Raghavachari Nalini N Woodhouse Kimberly K Yu Guoying G Barge Suchitra S Coronnello Claudia C Benos Panayiotis V PV Kato Gregory J GJ Kaminski Naftali N Gladwin Mark T MT

PloS one 20130412 4

<h4>Background</h4>Increased platelet activation in sickle cell disease (SCD) contributes to a state of hypercoagulability and confers a risk of thromboembolic complications. The role for post-transcriptional regulation of the platelet transcriptome by microRNAs (miRNAs) in SCD has not been previously explored. This is the first study to determine whether platelets from SCD exhibit an altered miRNA expression profile.<h4>Methods and findings</h4>We analyzed the expression of miRNAs isolated from ...[more]

PMID: 23593351

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Gene expression data in Meg-01 cells following transfection with platelet-enriched microRNA-1225-3p

Project description:Platelets from sickle cell disease patients have differentially expressed microRNAs as compared to platelets from healthy control subjects. The objective of this study is to overexpress an upregulated miRNA,miR-1225-3p, in MEG01 cells to identify putative targets.

2013-06-20 | GSE41573 | GEO

Transcription profiling of human chronic lymphocytic leukemia samples and pRS-15/16 transfected MEG01 cell lines to identify MiR-15a and miR-16-1 cluster function in human leukemia

Project description:Two samples obtained from the MEG01 cell line transfected with pRS-15/16 and pRS-E vector, each one in triplicate, and 16 CLL samples were analyzed by microarray using Human Genome U133A Plus 2.0 GeneChip arrays (Affymetrix).

2008-06-01 | E-MEXP-1482 | biostudies-arrayexpress

Identification of GATA1 transcriptional regulation in the megakaryoblastic cell line Meg01

Project description:chIP-on-chip analysis of GATA1 in the megakaryoblastic cell line Meg01. The IP was obtained with a GATA1 C-terminus antibody. The control sample was a normal goat IgG pull down done side by side with the GATA1 (C-20) antibody using the same soluble chromatin sample from non-treated Meg-01 cells. A 2-chip microarray set (Agilent 240K) was used for the chIP-on-chip analysis.

2013-07-01 | E-GEOD-43018 | biostudies-arrayexpress

The RNA-binding protein ATXN2 controls cellular processes during megakaryopoiesis

Project description:Megakaryocytes accumulate mRNA during their maturation, which is required for the correct spatio-temporal production of cytoskeletal proteins, membranes and platelet-specific granules, and for the subsequent shedding of thousands of platelets per cell. Gene expression profiling identified the RNA binding protein ATAXIN2 (ATXN2) as a putative novel regulator of megakaryopoiesis. ATXN2 expression is high in CD34+/CD41+ megakaryoblasts and sharply decreases upon maturation to megakaryocytes. ATXN2 associates with DDX6 suggesting that it may mediate repression of mRNA translation during early megakaryopoiesis. Comparative transcriptome and proteome analysis on megakaryoid cells (MEG-01) with differential ATXN2 expression identified ATXN2 dependent gene expression of mRNA and protein involved in processes linked to coagulation. Mice deficient for Atxn2 did not display differences in bleeding times, but expression of key surface receptors on platelets, such as ITGB3 (carries the CD61 antigen) and CD31 (PECAM1), were deregulated and platelet aggregation was reduced upon specific triggers.

2020-05-26 | PXD015823 | Pride

Protein interactors of MEG-1 in C. elegans embryos

Project description:In animals with germ plasm, specification of the germline involves “germ granules”, cytoplasmic condensates that enrich maternal transcripts in the germline founder cells. In C. elegans embryos, P granules enrich maternal transcripts, but surprisingly P granules are not essential for germ cell fate specification. Here we have described a second condensate in the C. elegans germ plasm. Like canonical P-bodies found in somatic cells, “germline P-bodies” contain regulators of mRNA decapping and deadenylation and, in addition, the intrinsically-disordered proteins MEG-1 and MEG-2 and the TIS11-family RNA-binding protein POS-1. Embryos lacking meg-1 and meg-2 do not stabilize P-body components, miss-regulate POS-1 targets, miss-specify the germline founder cell, and do not develop a germline. Our findings suggest that specification of the germ line involves at least two distinct condensates that independently enrich and regulate maternal mRNAs in the germline founder cells.

2022-10-05 | PXD036610 | Pride

Role of platelet micrornas in sickle cell disease

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-06-20 | E-GEOD-41575 | biostudies-arrayexpress

Runx1 (Aml1) knockdown in AMkL Meg-01 cells

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2009-08-01 | E-GEOD-17311 | biostudies-arrayexpress

Transfer of Alarmins from Neutrophils to Platelets after Acute Myocardial Infarction

Project description:Platelets are central to the pathophysiology of myocardial infarction (MI). How the platelet proteome is altered during MI is unknown. We sought to describe changes in the platelet proteome at the time of MI and identify potential mechanisms. Platelets from patients experiencing ST-elevation myocardial infarction (STEMI) before and 3 days after treatment (n= 30), and a matched cohort of patients with severe stable coronary artery disease (CAD) prior to and 3 days after coronary artery bypass grafting (CABG, n=25) underwent quantitative proteomic analysis. Elevations of the alarmins S100A8 and S100A9 were detected at the time of STEMI compared with stable CAD (S100A8, FC = 2.00, FDR = 0.05; S100A9, FC = 2.28, FDR = 0.005). During STEMI, S100A8 mRNA and protein levels were correlated in platelets (R = 0.46, p = 0.012). To determine if protein synthesis occurs, activated platelets were incubated with 13C-labelled amino acids for 24 hours and analyzed by mass spectrometry. No incorporation was detected, consistent with the notion that protein synthesis in platelets is not a major contributor to the platelet proteome. Platelet abundance of S100A8 and A9 was strongly correlated with neutrophil abundance at the time of STEMI. When isolated platelets and neutrophils were co-incubated under quiescent and TRAP-6 activated conditions, release of S100A8 from neutrophils resulted in uptake of S100A8 by platelets. Leukocyte-to-platelet protein transfer, rather than protein synthesis, may occur in a thromboinflammatory environment such as STEMI, representing a potential new contributor in the innate immune pathogenesis of STEMI.

2023-11-15 | PXD024159 | Pride

Homo sapiens

Project description:Gene expression data in Meg-01 cells following transfection with platelet-enriched microRNA-1225-3p

| PRJNA177572 | ENA

Gene expression profiling of MDA-MB-231 cells transfected with hsa-miR-205-5p and hsa-miR-214-3p compared to scrambled miRNA precursors

Project description:We have employed whole genome microarray to identify changes in gene expression in MDA-MB-231 cells transfected with hsa-miR-205-5p and hsa-miR-214-3p compared to scrambled miRNA precursors at 72 hours after transfection.

2020-05-31 | GSE148847 | GEO

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