Unknown,Transcriptomics,Genomics,Proteomics

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GRO-seq of Drosophila embryos at 2-2.5 hours and 3-3.5 hours after egg laying (AEL)


ABSTRACT: The transition in developmental control from maternal to zygotic gene products marks a critical step in early embryogenesis. Here, we use GRO-seq analysis to map the genome-wide RNA polymerase distribution during the Drosophila maternal to zygotic transition. This analysis unambiguously identifies the zygotic transcriptome, and provides insight into its mechanisms of regulation. Two replicates of GRO-seq at each time point.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Abbie Saunders 

PROVIDER: E-GEOD-41611 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Extensive polymerase pausing during Drosophila axis patterning enables high-level and pliable transcription.

Saunders Abbie A   Core Leighton J LJ   Sutcliffe Catherine C   Lis John T JT   Ashe Hilary L HL  

Genes & development 20130501 10


Cascades of zygotic gene expression pattern the anterior-posterior (AP) and dorsal-ventral (DV) axes of the early Drosophila embryo. Here, we used the global run-on sequencing assay (GRO-seq) to map the genome-wide RNA polymerase distribution during early Drosophila embryogenesis, thus providing insights into how genes are regulated. We identify widespread promoter-proximal pausing yet show that the presence of paused polymerase does not necessarily equate to direct regulation through pause rele  ...[more]

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