Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptomic profiling in childhood H1N1/09 influenza reveals reduced expression of protein synthesis genes


ABSTRACT: In order to understand the immunopathogenesis of severe influenza H1N1/09, we compared the whole blood RNA transcriptome of children hospitalised with H1N1/09 infection with that of children hospitalised with RSV or bacterial infection Blood was collected into PAX gene tubes (PreAnalytiX). Total RNA integrity was assessed using an Agilent 2100 Bioanalyzer (Agilent, Palo Alto, CA). Labeled cRNA was hybridized to Illumina Human HT-12 v3 Beadchips. Observational, prospective study. Patients were recruited at or after presentation to hospital. Main inclusion criteria were presence of illness of sufficient severity to warrant blood tests for clinical reasons, in child aged <17 years. Patient numbers recruited were as follows: H1N1/09 infection (n=25) RSV (n=34) (n=21), and healthy paediatric controls (n=33). After exclusion of children with co-infections, numbers of arrays analysed in were as follows: H1N1/09 n=19; RSV n=22; bacterial infection n=18; controls n=33

ORGANISM(S): Homo sapiens

SUBMITTER: Jethro Herberg 

PROVIDER: E-GEOD-42026 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptomic profiling in childhood H1N1/09 influenza reveals reduced expression of protein synthesis genes.

Herberg Jethro A JA   Kaforou Myrsini M   Gormley Stuart S   Sumner Edward R ER   Patel Sanjay S   Jones Kelsey D J KD   Paulus Stéphane S   Fink Colin C   Martinon-Torres Federico F   Montana Giovanni G   Wright Victoria J VJ   Levin Michael M  

The Journal of infectious diseases 20130729 10


We compared the blood RNA transcriptome of children hospitalized with influenza A H1N1/09, respiratory syncytial virus (RSV) or bacterial infection, and healthy controls. Compared to controls, H1N1/09 patients showed increased expression of inflammatory pathway genes and reduced expression of adaptive immune pathway genes. This was validated on an independent cohort. The most significant function distinguishing H1N1/09 patients from controls was protein synthesis, with reduced gene expression. R  ...[more]

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