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Transcriptomic difference caused by fpk1/2 kinase double deletion

ABSTRACT: Differential gene expression was determined using yeast cDNA microarray mRNA were enriched from auxotroph-matched yeast cells; control WT vs fpk1/2? cells and transcriptomic difference was examined BY4741 with HIS3 was compared with fpk1::KanMx fpk2::HIS3 cells in the log-phase. mRNA was enriched with mTRAP (Activemotief) from hot-phenol method total RNA fractions and was dual-color labeled with Cy-scribe (Amersham) to seek differentially expressed genes.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Hiromu Takematsu

PROVIDER: E-GEOD-42083 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Fpk1/2 kinases regulate cellular sphingoid long-chain base abundance and alter cellular resistance to LCB elevation or depletion.

Yamane-Sando Yukari Y Shimobayashi Etsuko E Shimobayashi Mitsugu M Kozutsumi Yasunori Y Oka Shogo S Takematsu Hiromu H

MicrobiologyOpen 20140210 2

Sphingolipids are a family of eukaryotic lipids biosynthesized from sphingoid long-chain bases (LCBs). Sphingolipids are an essential class of lipids, as their depletion results in cell death. However, acute LCB supplementation is also toxic; thus, proper cellular LCB levels should be maintained. To characterize the "sphingolipid-signaling intercross," we performed a kinome screening assay in which budding yeast protein kinase-knockout strains were screened for resistance to ISP-1, a potent inhi ...[more]

PMID: 24510621

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