Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq data of 16 strains of saccharomyces cerevisiae produced after knockout of all combination of HIS3, LEU2, URA3 and MET15 genes


ABSTRACT: In this study we have deleted four metabolic genes (HIS3, LEU2, URA3 and MET15) in their sixteen possible combinations. These strains are following: knockout of HIS3, LEU2, URA3, MET15; knockout of HIS3, LEU2, MET15; knockout of HIS3, URA3, MET15; knockout of LEU2, URA3, MET15; knockout of HIS3, LEU2, URA3; knockout of HIS3, MET15; knockout of LEU2, MET15; knockout of URA3, MET15; knockout of HIS3, LEU2; knockout of HIS3, URA3; knockout of LEU2, URA3; knockout of MET15, knockout of HIS3; knockout of LEU2; knockout of URA3 and prototrophic strain. We grew the 16 strains in synthetic complete media (SC), and sampled for transcriptomincs in triplicates at mid-exponential phase (OD value of 0.8).

INSTRUMENT(S): Illumina TruSeq-RNA-Kit, Illumina HiSeq 2000

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Mohammad Tauqeer Alam 

PROVIDER: E-MTAB-3991 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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