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Deriving Dopaminergic Neurons for Clinical Use. A Practical Approach.

ABSTRACT: Identification of new small molecules that regulate the step-wise differentiation of hPSC into dopaminergic neurons population. Furthermore, the naturally occurring steroid, guggulsterone, was found to be the most effective inducer of neural stem cells into dopaminergic neurons. Total RNA extracted at different stages of neural differentiation from human pluripotent stem cells

ORGANISM(S): Homo sapiens

SUBMITTER: Francesca Boscolo

PROVIDER: E-GEOD-42265 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deriving dopaminergic neurons for clinical use. A practical approach.

Gonzalez Rodolfo R Garitaonandia Ibon I Abramihina Tatiana T Wambua Gerald K GK Ostrowska Alina A Brock Mathew M Noskov Alexander A Boscolo Francesca S FS Craw John S JS Laurent Louise C LC Snyder Evan Y EY Semechkin Ruslan A RA

Scientific reports 20130101

New small molecules that regulate the step-wise differentiation of human pluripotent stem cells into dopaminergic neurons have been identified. The steroid, guggulsterone, was found to be the most effective inducer of neural stem cells into dopaminergic neurons. These neurons are extensively characterized and shown to be functional. We believe this new approach offers a practical route to creating neurons of sufficient quality to be used to treat Parkinson's disease patients. ...[more]

PMID: 23492920

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