Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Comparison of the transcriptome of the conditional afpig-a mutant and the wild-type

ABSTRACT: In order to gain a better understanding of the mechanism of increased cell death triggered by deficient GPI anchoring, we constructed a whole genome microarray using the Agilent eArray 5.0 program.The chip specification is 8M-CM-^W15K. The genome sequences were downloaded from the following site:http://www.ncbi.nlm.nih.gov/genome?Db=genome&Cmd=Search&Term=txid330879[orgn]. Each gene was represented by one 60-nt oligonucleotide probe, and 358 genes out of the 9630 total were replicated 14 times.Using this microarray,we got 3274 genes which were induced or reduced at least 1.5 fold in the afpig-a mutant comparing to the wild-type. Mycelia of the afpig-a mutant and the wild-type were harvested from strains grown in liquid CM at 37M-BM-0C for 36h with shaking at 200 rpm and ground by hand.Total RNA was extracted to do the hybridization.cRNAs were labled with Cy3 NHS ester.afpig-a_37 was sample, afpig_37_1a,afpig_37_2a,afpig_37_3a and afpig_37_4a were biological repeats. w_37 was control,w_37_1a,w_37_2a,w_37_3a and w_37_4a were biological repeats.Four independent experiments were performed. Each time we got a set of differentilly expressed genes.Differentially expressed genes were selected with PM-bM-^IM-$0.05, FCM-bM-^IM-%1.5 by T-test methods using the independent four sets data.

ORGANISM(S): Aspergillus fumigatus

SUBMITTER: jianghong yan

PROVIDER: E-GEOD-42499 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Transcriptome and biochemical analysis reveals that suppression of GPI-anchor synthesis leads to autophagy and possible necroptosis in Aspergillus fumigatus.

Yan Jianghong J Du Ting T Zhao Wan W Hartmann Thomas T Lu Hua H Lü Yang Y Ouyang Haomiao H Jiang Xuejun X Sun Lei L Jin Cheng C

PloS one 20130318 3

Previously, it has been shown that GPI proteins are required for cell wall synthesis and organization in Aspergillus fumigatus, a human opportunistic pathogen causing life-threatening invasive aspergillosis (IA) in immunocompromised patients. Blocking GPI anchor synthesis leads to severe phenotypes such as cell wall defects, increased cell death, and attenuated virulence. However, the mechanism by which these phenotypes are induced is unclear. To gain insight into global effects of GPI anchoring ...[more]

PMID: 23527074

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Dataset Information

Comparison of the transcriptome of the conditional afpig-a mutant and the wild-type

Publications

Transcriptome and biochemical analysis reveals that suppression of GPI-anchor synthesis leads to autophagy and possible necroptosis in Aspergillus fumigatus.

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