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Gene expression changes in embryonic stem cells in response to Tcf15-E47

ABSTRACT: We set out to identify genes that respond rapidly to overexpression of a Tcf15-E47 heterodimeric transcription factor in mouse embryonic stem cells. Dox inducible Tcf15-E47 mouse embryonic stem cells were exposed to dox for 0, 8, 12, 24h and samples collected in duplicate. Control parental cells were exposed to dox for 0 or 8 h and samples collected in duplicate. Total 12 samples.

ORGANISM(S): Mus musculus

SUBMITTER: Sally Lowell

PROVIDER: E-GEOD-42539 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Tcf15 primes pluripotent cells for differentiation.

Davies Owen R OR Lin Chia-Yi CY Radzisheuskaya Aliaksandra A Zhou Xinzhi X Taube Jessica J Blin Guillaume G Waterhouse Anna A Smith Andrew J H AJ Lowell Sally S

Cell reports 20130207 2

The events that prime pluripotent cells for differentiation are not well understood. Inhibitor of DNA binding/differentiation (Id) proteins, which are inhibitors of basic helix-loop-helix (bHLH) transcription factor activity, contribute to pluripotency by blocking sequential transitions toward differentiation. Using yeast-two-hybrid screens, we have identified Id-regulated transcription factors that are expressed in embryonic stem cells (ESCs). One of these, Tcf15, is also expressed in the embry ...[more]

PMID: 23395635

Similar Datasets

Gene expression changes in embryonic stem cells in response to Tcf15-E47

Project description:We set out to identify genes that respond rapidly to overexpression of a Tcf15-E47 heterodimeric transcription factor in mouse embryonic stem cells.

2013-02-08 | GSE42539 | GEO

Identification of E47 targets

2006-05-09 | GSE4784 | GEO

Identification of E47 targets

Project description:In maturing T-lineage cells, the helix-loop-helix protein E47 has been shown to enforce a critical proliferation and developmental checkpoint commonly referred to as Î² selection. To examine how E47 regulates cellular expansion and developmental progression, we have used an E2A deficient lymphoma cell line and DNA microarray analysis to identify immediate E47 target genes. Hierarchical cluster analysis of gene expression patterns revealed that E47 coordinately regulates the expression of genes involved in cell survival, cell growth, lipid metabolism, stress response and lymphoid maturation. These include Cdk6, CD25, Tox, Gadd45a, Gadd45b, Gfi1, Gfi1b, Socs1, Socs3, Id2, Eto2 and Xbp1. We propose a regulatory network linking JAK/STAT mediated signaling, E47 and SOCS proteins in a common pathway. Finally, we suggest that the aberrant activation of Cdk6 in E47 deficient T-lineage cells contributes to the development of lymphoid malignancy. We used the 1F9 cell line, originally isolated from E2A deficient thymomas. To identify E47 target genes, 1F9 cells were transduced with a retrovirus carrying an E47/estrogen receptor (E47ER) hybrid protein. Control cells were transduced with virus carrying the bHLH region but lacking the N-terminal transactivation domain. Both retroviral constructs also directed the expression of human CD25 (hCD25) to allow rapid isolation of transduced cells. One day post-infection, cells were incubated with 4-hydroxytamoxifen (4-OHT) for a period of six hours, to activate the E47ER fusion protein. hCD25 positive cells were purified using magnetic beads, RNA was isolated and used to generate probes for hybridization to murine oligonucleotide microarrays.

2010-07-01 | E-GEOD-4784 | biostudies-arrayexpress

Genes regulated by E47

Project description:To find the target genes regulated by E47 in hematopoietic progenitor cells Keywords: genetic modification

2006-04-22 | GSE4693 | GEO

E-cadherin and EMT regulation by E47 transcription represor

Project description:Expression profile analysis in MDCK-E47 cells in comparisom with MDCK CMV control cells Keywords: Genetic Modification (overexpression of E-cadherin repressors) The Oncochip microarray platform v1.1 contains 9,726 clones corresponding to 6,386 different genes, and it includes 2,489 duplicate clones. Duplicate samples (different extrations of RNA) were labeled with dUTP-Cy5 and hybridized against dUTP-Cy3-MDCK control cells. In addittion, one RNA extraction was labelled with dUTP-Cy3 and was hybridized againts dUTP-Cy5- CMV control cells

2010-06-24 | E-GEOD-9147 | biostudies-arrayexpress

ChIP-MS for GR in liver from wild type and E47 mutant mice

Project description:Glucocorticoids (GCs) are widely prescribed effective drugs, but their clinical use is compromised by severe side effects including hyperglycemia, hyperlipidemia and obesity. They bind to the Glucocorticoid Receptor (GR), which acts as a ligand-gated transcription factor. The transcriptional activation of metabolic genes by GR is thought to underlie these undesired adverse effects. Using mouse genetics, ChIP-Seq, RNA-Seq and ChIP-MS, we found that the bHLH transcription factor E47 is required for the regulation of hepatic glucose and lipid metabolism by GR in vivo, and that loss of E47 prevents the development of hyperglycemia and hepatic steatosis in response to GCs. Here we show that E47 and GR co-occupy metabolic promoters and enhancers. E47 is needed for the efficient binding of GR to chromatin and for the adequate recruitment of coregulators such as Mediator. Taken together, our results illustrate how GR and E47 regulate hepatic metabolism, and how inhibition of E47 might provide an entry point for novel GC therapies with reduced side effect profiles. These ChIP-MS data sets show IPs for GR in both wildtype and E47 mutant mouse livers treated with the synthetic glucocorticoid Dexamethasone.

2018-11-22 | PXD010157 | Pride

Expression data of in vitro generated regulatory T cells overexpressing E47

Project description:E47 represses Foxp3 transcription, albeit indirectly through the activation of unknown negative regulatory of Foxp3 transcription. To identify target genes of E47 in regulatory T cells, we compared gene expression profiles of Treg cells overexpressing E47 compared to control-vector transduced cells.

2016-12-06 | GSE59557 | GEO

E47 KO versus WT HSCs

Project description:Genome-wide gene expression pattern of E47 KO versus WT HSCs from primary and secondary recipient mice were analysis using Agilent one-color micro-array analysis.

2011-01-22 | GSE26788 | GEO

Gene expression analysis of PANC-1 cells inducibly expressing the bHLH protein E47

Project description:Analysis of induced E47 expression on PDA cells at the gene expression level.The hypothesis tested in the present study was that PDA cells can be reprogrammed to revert to their original quiescent acinar cell phenotype by stimulating a tamoxifen inducible form of E47 fused to a modified estrogen (E47-ER) receptor . Results provide important information on the remarkable ability of E47ER to trigger reactivation of the acinar cell differentiation program and cell cycle arrest in PDA cells.

2015-04-20 | GSE55999 | GEO

Genes regulated by E47

Project description:To find the target genes regulated by E47 in hematopoietic progenitor cells E2A-deficient hematopoietic progenitor cells were infected by E47ER or bHLHER. 48 hours later, the cells were induced by 4-hydroxytamoxifen for six hours.

2010-07-01 | E-GEOD-4693 | biostudies-arrayexpress

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