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Annexin A2 Modulates Radiation-Sensitive Transcriptional Programming and Cell Fate

ABSTRACT: Whole genome expression profiling in the presence and absence of annexin A2 [shRNA] identified fundamentally altered transcriptional programming that changes the radioresponsive transcriptome. Bioinformatics predicted that silencing AnxA2 may enhance cell death responses to stress in association with reduced activation of pro-survival signals such as nuclear factor kappa B. This prediction was validated by demonstrating a significant increase in sensitivity toward tumor necrosis factor alpha induced cell death in annexin A2 silenced cells, relative to vector controls, associated with reduced nuclear translocation of RelA (p65) following tumor necrosis factor alpha treatment. Murine progenitor cells, JB6 cell line, were stably transformed with Annexin A2 shRNA or vector only control and then treated with ionizing radiation at two doses, 10cGy and 100cGy. Samples were collected at 2hr, 6hr and 24hr for RNA isolation. Two untreated time points were collected also: 2hr and 24hr.

ORGANISM(S): Mus musculus

SUBMITTER: Katrina Waters

PROVIDER: E-GEOD-42547 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Annexin A2 Modulates Radiation-Sensitive Transcriptional Programming and Cell Fate

Project description:Whole genome expression profiling in the presence and absence of annexin A2 [shRNA] identified fundamentally altered transcriptional programming that changes the radioresponsive transcriptome. Bioinformatics predicted that silencing AnxA2 may enhance cell death responses to stress in association with reduced activation of pro-survival signals such as nuclear factor kappa B. This prediction was validated by demonstrating a significant increase in sensitivity toward tumor necrosis factor alpha induced cell death in annexin A2 silenced cells, relative to vector controls, associated with reduced nuclear translocation of RelA (p65) following tumor necrosis factor alpha treatment.

2012-11-28 | GSE42547 | GEO

Expression data of J3T-1, J3T-2, J3T-1shA, J3T-2A glioma

Project description:We have previously established two sibling glioma subclones, J3T-1 and J3T-2, showing distinct invasive and angiogenic phenotypes. J3T-1, expressing high annexin A2, demonstrates robust angiogenesis and tumor invasion around neovasculature. J3T-2, expressing low annexin A2, demonstrates diffuse invasion along white matter tracts. Knockdown of annexin A2 in J3T-1 (J3T-1shA) resulted in diffuse invasion pattern, and overexpression of annexin A2 in J3T-2 (J3T-2A) showed prominent angiogenesis. We used microarrays to identify genes which promote the phenotypic transition regulated by annexin A2.

2020-04-20 | GSE138374 | GEO

Chronic low dose-rate versus acute ionizing radiation induced gene expression in primary derived prostate fibroblasts

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Binding partners of Annexin A2 in MDA-MB-231 cells

Project description:A mass-spec of proteins found bound to Annexin A2 following a immunoprecipitation of Annexin A2 from cell lysate of MDAMB231 cells plated 1) on plastic or 2) on collagen-1

2023-10-11 | MSV000093084 | MassIVE

Global analysis of the impact of environmental perturbation on cis-regulation of gene expression

Project description:This SuperSeries is composed of the following subset Series: GSE21410: Global analysis of the impact of environmental perturbation on cis-regulation of gene expression: BMP2, 2hr GSE21725: Global analysis of the impact of environmental perturbation on cis-regulation of gene expression: DEX, 24hr GSE21726: Global analysis of the impact of environmental perturbation on cis-regulation of gene expression: PGE2, 24hr GSE21727: Global analysis of the impact of environmental perturbation on cis-regulation of gene expression: control and variety of treatments, 2hr and 24hr Refer to individual Series

2010-10-01 | E-GEOD-21728 | biostudies-arrayexpress

Differential Regulation of Mitogen-Activated Protein Kinases by Acetaminophen in TAMH cells

Project description:Acetaminophen (APAP), a widely used analgesic and antipyretic that is considered to be relatively safe at recommended doses, is the leading cause of drug-induced liver failure in the United States. 3M-bM-^@M-^Y-Hydroxyacetanilide (AMAP), a regioisomer of acetaminophen is useful as a comparative tool for studying APAP-induced toxicity since it is non-toxic relative to APAP. TGF-alpha transgenic mouse hepatocytes were treated with both isomers to investigate mitogen-activated protein kinase cascades in order to differentiate their toxicological outcomes. Mitogen-activated protein kinase (MAPK) cascade expression and activation were measured using microarray and Bioplex technologies, respectively. APAP treatment led to c-Jun N-terminal kinase (JNK) activation, whereas AMAP treatment led to the activation of extracellular-signal-regulated protein kinase (ERK). The microarray data suggested APAP treatment may upregulate gene expression at multiple levels of the JNK cascade including a JNK-related scaffold protein. Expression data was related to phosphoprotein levels using the Bioplex system. APAP treatment led to a significant activation of JNK compared to its regioisomer. In contrast, microarray analysis of AMAP showed a slight upregulation of ERK gene activity. Furthermore, Bioplex data showed AMAP treatment led to significant ERK phosphorylation compared to APAP. Cell viability assays confirmed that APAP-induced activation of JNK was related to higher rates of cell death, whereas activation of ERK by AMAP may be cytoprotective. 27 arrays, 9 experimental groups, 2hr AMAP, 2hr APAP, 2hr Control, 6hr AMAP, 6hr APAP, 6hr Control, 24hr AMAP, 24hr APAP, 24hr Control.

2010-07-06 | E-GEOD-18614 | biostudies-arrayexpress

Project description:Immature (19/20 days of age) Alpk:APfCD-1 mice were treated with arachis oil (AO) vehicle, 0.4mg/kg 17beta-estradiol (E2), or 250mg/kg genistein (GEN), via a single subcutaneous injection, and sacrificed at 1hr, 2hr, 4hr, 8hr, 24hr, 48hr, 72hr post dose. Keywords: other

2004-10-06 | GSE1819 | GEO

Chronic low dose-rate versus acute ionizing radiation induced gene expression in primary derived prostate fibroblasts

Project description:Using a novel flood source construct, we performed a direct comparison of genome-wide gene expression regulations resulting from exposure of primary human prostate fibroblast cultures to acute (10cGy and 200cGy) and longer-term chronic (1.0-2.45cGy cumulative over 24hr) exposures. Samples were collected at 24 hours after an acute 10cGy or 200cGy exposure (48cGy per minute) versus immediately after an accumulated 24 hour chronic 1.0 to 2.45cGy low dose-rate exposure (7 to 17M-BM-5Gy per minute). Control 0cGy samples were collected at the same time. A total of 4 independent experimental replicates were used for each sample resulting in (2 individuals M-CM-^W 4 experimental conditions M-CM-^W 4 experimental replicates) 32 microarrays.

2013-12-20 | E-GEOD-52918 | biostudies-arrayexpress

Mus musculus

Project description:Role of annexin A2 in muscle repair

| PRJNA345192 | ENA

Phenotypic Anchoring of Gene Expression Changes during Estrogen-Induced Uterine Growth

Project description:Immature (19/20 days of age) Alpk:APfCD-1 mice were treated with arachis oil (AO) vehicle or 0.4mg/kg 17beta-estradiol (E2), via a single subcutaneous injection, and sacrificed at 1hr, 2hr, 4hr, 8hr, 24hr, 48hr, 72hr post dose. Keywords = estrogen Keywords = gene expression Keywords = microarray Keywords = phenotypic anchoring Keywords = uterus Keywords: time-course

2005-01-28 | GSE2195 | GEO

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