Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene expression profiles of MCF7 with with the following NR ligands: estradiol/E2 (ERα agonist), AM580 (RARA agonist), CD437 (RARG agonist), ATRA (RARA/RARG agonist), GW0742 (PPARD agonist), R5020 (PGR agonist), and dexamethasone (GR/NR3C1 agonist).


ABSTRACT: Altered gene expression patterns in human diseases reflect perturbations in the transcriptional networks that regulate cellular state. In breast cancer, Nuclear Receptors (NRs) play a prominent role in governing gene expression. NRs have prognostic utility and are therapeutically important targets. In order to understand the function of nuclear receptors in breast cancer, we treated MCF7 cells with 6 nuclear receptor agonist, and performed expression arrrays with three biological replicates each. MCF-7 cells were cultured for 48 hours in medium with 10% charcoal-stripped FBS, and then were treated with 6 diffferent NR ligand (E2, AM580, CD437, ATRA, R5020, DEX) separately for 24h before collection and processing for microarrays. Three biological replicates were collected and processed for both control and treatment. with the 100nM GW0742 (PPARD specific ligand) and 100nM ATRA (RARs ligand), then collected after 24h for microarray processing. Controls were non-treated MCF7 cells. Both treatments and controls have three biological replicates.

ORGANISM(S): Homo sapiens

SUBMITTER: Jie Zhou 

PROVIDER: E-GEOD-42619 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

A comprehensive nuclear receptor network for breast cancer cells.

Kittler Ralf R   Zhou Jie J   Hua Sujun S   Ma Lijia L   Liu Yuwen Y   Pendleton Elisha E   Cheng Chao C   Gerstein Mark M   White Kevin P KP  

Cell reports 20130131 2


In breast cancer, nuclear receptors (NRs) play a prominent role in governing gene expression, have prognostic utility, and are therapeutic targets. We built a regulatory map for 24 NRs, six chromatin state markers, and 14 breast-cancer-associated transcription factors (TFs) that are expressed in the breast cancer cell line MCF-7. The resulting network reveals a highly interconnected regulatory matrix where extensive crosstalk occurs among NRs and other breast -cancer-associated TFs. We show that  ...[more]

Similar Datasets

2013-01-01 | GSE42619 | GEO
2023-07-20 | GSE213793 | GEO
2023-07-20 | GSE213742 | GEO
2023-01-11 | GSE199381 | GEO
2019-12-18 | PXD008761 | Pride
2023-07-20 | PXD041749 | Pride
2023-07-20 | PXD040953 | Pride
2015-08-01 | E-GEOD-59995 | biostudies-arrayexpress
2021-09-09 | PXD020969 | Pride
2013-07-02 | GSE47958 | GEO