Unknown,Transcriptomics,Genomics,Proteomics

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Effect of cigarette smoke on gene expression in mammary epithelial cells [gene expression]


ABSTRACT: Recent epidemiological studies demonstrate that both active and involuntary exposure to tobacco smoke increases the risk of breast cancer. Little is known, however, about the molecular mechanisms by which tobacco smoke contributes to breast carcinogenesis. To investigate these mechanisms we have analyzed gene expression and methylation in MCF 10A mammary epithelial cells chronically exposed to aqueous cigarette smoke extract (CSE). CSE was prepared weekly and added to the cell cultures at a concentration equivalent to 0.001 cigarettes/ml. Two clones were isolated after 13 weeks of treatment and expanded in the same concentration of CSE for 8 additional weeks. Mock-treated samples were prepared in parallel.

ORGANISM(S): Homo sapiens

SUBMITTER: Francescopaolo Di Cello 

PROVIDER: E-GEOD-42667 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Cigarette smoke induces epithelial to mesenchymal transition and increases the metastatic ability of breast cancer cells.

Di Cello Francescopaolo F   Flowers V Lynn VL   Li Huili H   Vecchio-Pagán Briana B   Gordon Brent B   Harbom Kirsten K   Shin James J   Beaty Robert R   Wang Wei W   Brayton Cory C   Baylin Stephen B SB   Zahnow Cynthia A CA  

Molecular cancer 20130806


<h4>Background</h4>Recent epidemiological studies demonstrate that both active and involuntary exposure to tobacco smoke increase the risk of breast cancer. Little is known, however, about the molecular mechanisms by which continuous, long term exposure to tobacco smoke contributes to breast carcinogenesis because most previous studies have focused on short term treatment models. In this work we have set out to investigate the progressive transforming effects of tobacco smoke on non-tumorigenic  ...[more]

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