Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Yiting Yu
PROVIDER: E-GEOD-42723 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Bhattacharyya Sanchari S Yu Yiting Y Suzuki Masako M Campbell Nathaniel N Mazdo Jozef J Vasanthakumar Aparna A Bhagat Tushar D TD Nischal Sangeeta S Christopeit Maximilian M Parekh Samir S Steidl Ulrich U Godley Lucy L Maitra Anirban A Greally John M JM Verma Amit A
Nucleic acids research 20130716 16
5-hydroxymethylcytosine (5-hmC) is a recently discovered epigenetic modification that is altered in cancers. Genome-wide assays for 5-hmC determination are needed as many of the techniques for 5-methylcytosine (5-mC) determination, including methyl-sensitive restriction digestion and bisulfite sequencing cannot distinguish between 5-mC and 5-hmC. Glycosylation of 5-hmC residues by beta-glucosyl transferase (β-GT) can make CCGG residues insensitive to digestion by MspI. Restriction digestion by H ...[more]