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Gene expression changes in subclones of a Burkitt lymphoma cell line with different patterns of EBV latent infection

ABSTRACT: In this study we have investigated the gene expression profiles of three different types of subclone all generated by single cell cloning of the same parental EBV positive Burkitt lymphoma cell line Awia-BL. These included EBV negative clones which have lost the virus episome, EBV positive clones with a conventional Latency I form of infection and EBV positive clones with an atypical Wp-restricted form of infection. Gene expression was compared between two different EBV negative clones (both performed in biological duplicate), four different Latency I clones and four different Wp-restricted clones using HG U133 Plus 2.0 gene chip arrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Wenbin Wei

PROVIDER: E-GEOD-42867 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Different patterns of Epstein-Barr virus latency in endemic Burkitt lymphoma (BL) lead to distinct variants within the BL-associated gene expression signature.

Kelly Gemma L GL Stylianou Julianna J Rasaiyaah Jane J Wei Wenbin W Thomas Wendy W Croom-Carter Deborah D Kohler Christian C Spang Rainer R Woodman Ciaran C Kellam Paul P Rickinson Alan B AB Bell Andrew I AI

Journal of virology 20121226 5

Epstein-Barr virus (EBV) is present in all cases of endemic Burkitt lymphoma (BL) but in few European/North American sporadic BLs. Gene expression arrays of sporadic tumors have defined a consensus BL profile within which tumors are classifiable as "molecular BL" (mBL). Where endemic BLs fall relative to this profile remains unclear, since they not only carry EBV but also display one of two different forms of virus latency. Here, we use early-passage BL cell lines from different tumors, and BL s ...[more]

PMID: 23269792

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