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Unknown,Transcriptomics,Genomics,Proteomics

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Global microRNA profiling of aTC1-6 and bTC1 before and after treatment with cytokines

ABSTRACT: The study sought to determine the global miRNA profiles of mouse pancreatic cell lines aTC1-6 and bTC1 at steady state and after treatment with a cocktail of pro-inflammatory cytokines (IL1b; IFNg and TNFa) for a time course of 24 and 48 hours. Inflammatory cocktail contains IL1b 50 IU/ml; IFNg 1000 IU/ml; TNFa 1000 IU/ml. MicroRNA expression profiles in both cell lines during the different experimental conditions were determined using TaqManM-BM-. Rodent miRNA A+B Cards Set v2.0 (TLDA, Life Technologies). MicroRNA profiles were measured in 3 independent biological replicates. qRT-PCR analysis of microRNAs of aTC1-6 treated with cytokines for 24 and 48 h and of their respective not-treated controls as well as of M-NM-2TC1 cells not treated with cytokines. 180 ng RNA of each sample was reverse transcribed through MegaplexM-bM-^DM-" RT Rodent Primer Pool sets A and B, and preamplified through MegaplexM-bM-^DM-" PreAmp Rodent Primer Pool sets (LifetechnologiesM-bM-^DM-"). Complementary DNA (cDNA) was amplified usingTaqManM-BM-. Rodent miRNA A+B Cards Set v2.0 (Life TechnologiesM-bM-^DM-") with TaqMan Universal PCR Master Mix on an ABI 7900HT Sequence Detection System.

ORGANISM(S): Mus musculus

SUBMITTER: Michele Purrello

PROVIDER: E-GEOD-42970 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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