Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Resveratrol-Induced Gene Expression Profiles in Human Prostate Cancer Cells

ABSTRACT: Cell Culture and Treatments The LNCaP cell line was obtained from American Type Culture Collection (Manassas, VA) and maintained in RPMI medium with 10% fetal bovine serum (CDT; Hyclone Laboratories, Logan, UT) and penicillin/streptomycin (Mediatech, Herndon, VA) in an environment of 95% air and 5% CO2 at 370C. Upon reaching 75% confluence, cells were treated with either DMSO control or purified resveratrol at several concentrations (LKT Laboratories, St. Paul, MN), dissolved in DMSO, and incubated for varying lengths of time. Final concentration of DMSO in media was 0.01%. Total RNA was prepared from cells using TRIzol (Invitrogen Life Technologies, Carlsbad, CA). An all pairs experiment design type is where all labeled extracts are compared to every other labeled extract. Computed

ORGANISM(S): Homo sapiens

SUBMITTER: Sunita Jones

PROVIDER: E-GEOD-4299 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Resveratrol-induced gene expression profiles in human prostate cancer cells.

Jones Sunita B SB DePrimo Samuel E SE Whitfield Michael L ML Brooks James D JD

Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 20050301 3

<h4>Objective</h4>The transhydroxystilbene resveratrol is found at high levels in red wine and grapes, and red wine consumption may be inversely associated with prostate cancer risk. To gain insights into the possible mechanisms of action of resveratrol in human prostate cancer, we did DNA microarray analysis of the temporal transcriptional program induced by treatment of the human prostate cancer cell line LNCaP with resveratrol.<h4>Methods</h4>Spotted DNA microarrays containing over 42,000 ele ...[more]

PMID: 15767336

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Dataset Information

Resveratrol-Induced Gene Expression Profiles in Human Prostate Cancer Cells

Publications

Resveratrol-induced gene expression profiles in human prostate cancer cells.

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