Unknown,Transcriptomics,Genomics,Proteomics

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Diverse and specific gene expression responses to stresses in cultured human cells.

ABSTRACT: TT3 and TN are cell cycle time courses and should be centered or transformed before clustering or computing fourier scores. The preferred method is SVD centering preceded by KNNImpute missing-value estimation, although mean-centering is a viable alternative. Hela Heat 42 degrees time course has 2 zeroes (the second is labeled "Cold 25 degrees 0h"). Normalization is by centering or zero transformation (average 2 zeroes) WI38 Crowding (1-3 days) was by plating cells at ~50%? confluence and allowing to age. The zeroes are the same as for WI38 Heat Shock (there are 2) Other time courses should be self-explanatory. They have 1-4 zeroes each and can be treated either by centering or zero-transforming. WI38 Set of arrays organized by shared biological context, such as organism, tumors types, processes, etc. Keywords: Logical Set Computed

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Whitfield

PROVIDER: E-GEOD-4301 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Diverse and specific gene expression responses to stresses in cultured human cells.

Murray John Isaac JI Whitfield Michael L ML Trinklein Nathan D ND Myers Richard M RM Brown Patrick O PO Botstein David D

Molecular biology of the cell 20040305 5

We used cDNA microarrays in a systematic study of the gene expression responses of HeLa cells and primary human lung fibroblasts to heat shock, endoplasmic reticulum stress, oxidative stress, and crowding. Hierarchical clustering of the data revealed groups of genes with coherent biological themes, including genes that responded to specific stresses and others that responded to multiple types of stress. Fewer genes increased in expression after multiple stresses than in free-living yeasts, which ...[more]

PMID: 15004229

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Diverse and specific gene expression responses to stresses in cultured human cells.

Publications

Diverse and specific gene expression responses to stresses in cultured human cells.

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